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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylibium petroleiphilum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP384737MNX
CSB-EP384737MNX
CSB-BP384737MNX
CSB-MP384737MNX
CSB-EP384737MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Urease accessory protein UreD (ureD) CSB-YP384738MNX
CSB-EP384738MNX
CSB-BP384738MNX
CSB-MP384738MNX
CSB-EP384738MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum ATP phosphoribosyltransferase (hisG) CSB-YP384739MNX
CSB-EP384739MNX
CSB-BP384739MNX
CSB-MP384739MNX
CSB-EP384739MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP384740MNX
CSB-EP384740MNX
CSB-BP384740MNX
CSB-MP384740MNX
CSB-EP384740MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Sec-independent protein translocase protein TatB (tatB), partial CSB-YP384741MNX
CSB-EP384741MNX
CSB-BP384741MNX
CSB-MP384741MNX
CSB-EP384741MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Undecaprenyl-diphosphatase (uppP), partial CSB-YP384742MNX1
CSB-EP384742MNX1
CSB-BP384742MNX1
CSB-MP384742MNX1
CSB-EP384742MNX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP384743MNX
CSB-EP384743MNX
CSB-BP384743MNX
CSB-MP384743MNX
CSB-EP384743MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP384744MNX
CSB-EP384744MNX
CSB-BP384744MNX
CSB-MP384744MNX
CSB-EP384744MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP384745MNX
CSB-EP384745MNX
CSB-BP384745MNX
CSB-MP384745MNX
CSB-EP384745MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Probable transcriptional regulatory protein Mpe_A1337 (Mpe_A1337) CSB-YP384746MNX
CSB-EP384746MNX
CSB-BP384746MNX
CSB-MP384746MNX
CSB-EP384746MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Probable Fe (2+)-trafficking protein CSB-YP384747MNX
CSB-EP384747MNX
CSB-BP384747MNX
CSB-MP384747MNX
CSB-EP384747MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP384748MNX
CSB-EP384748MNX
CSB-BP384748MNX
CSB-MP384748MNX
CSB-EP384748MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP384749MNX
CSB-EP384749MNX
CSB-BP384749MNX
CSB-MP384749MNX
CSB-EP384749MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP384750MNX1
CSB-EP384750MNX1
CSB-BP384750MNX1
CSB-MP384750MNX1
CSB-EP384750MNX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribulose bisphosphate carboxylase large chain 1 (cbbL1) CSB-YP384751MNX
CSB-EP384751MNX
CSB-BP384751MNX
CSB-MP384751MNX
CSB-EP384751MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S6 (rpsF) CSB-YP384752MNX
CSB-EP384752MNX
CSB-BP384752MNX
CSB-MP384752MNX
CSB-EP384752MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Biotin synthase (bioB) CSB-YP384753MNX
CSB-EP384753MNX
CSB-BP384753MNX
CSB-MP384753MNX
CSB-EP384753MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum LexA repressor (lexA) CSB-YP384754MNX
CSB-EP384754MNX
CSB-BP384754MNX
CSB-MP384754MNX
CSB-EP384754MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L20 (rplT) CSB-YP384755MNX
CSB-EP384755MNX
CSB-BP384755MNX
CSB-MP384755MNX
CSB-EP384755MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribosome-binding factor A (rbfA) CSB-YP384756MNX
CSB-EP384756MNX
CSB-BP384756MNX
CSB-MP384756MNX
CSB-EP384756MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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