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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei Ribonuclease PH (rph) CSB-YP386618BPQ
CSB-EP386618BPQ
CSB-BP386618BPQ
CSB-MP386618BPQ
CSB-EP386618BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Serine--tRNA ligase (serS) CSB-YP386619BPQ
CSB-EP386619BPQ
CSB-BP386619BPQ
CSB-MP386619BPQ
CSB-EP386619BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Undecaprenyl-diphosphatase (uppP), partial CSB-YP386620BPQ1
CSB-EP386620BPQ1
CSB-BP386620BPQ1
CSB-MP386620BPQ1
CSB-EP386620BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease subunit gamma (ureA) CSB-YP386621BPQ
CSB-EP386621BPQ
CSB-BP386621BPQ
CSB-MP386621BPQ
CSB-EP386621BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP386622BPQ
CSB-EP386622BPQ
CSB-BP386622BPQ
CSB-MP386622BPQ
CSB-EP386622BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP386623BPQ
CSB-EP386623BPQ
CSB-BP386623BPQ
CSB-MP386623BPQ
CSB-EP386623BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaJ (dnaJ) CSB-YP386624BPQ
CSB-EP386624BPQ
CSB-BP386624BPQ
CSB-MP386624BPQ
CSB-EP386624BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP386625BPQ
CSB-EP386625BPQ
CSB-BP386625BPQ
CSB-MP386625BPQ
CSB-EP386625BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP386626BPQ
CSB-EP386626BPQ
CSB-BP386626BPQ
CSB-MP386626BPQ
CSB-EP386626BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP386627BPQ1
CSB-EP386627BPQ1
CSB-BP386627BPQ1
CSB-MP386627BPQ1
CSB-EP386627BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP386628BPQ
CSB-EP386628BPQ
CSB-BP386628BPQ
CSB-MP386628BPQ
CSB-EP386628BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP386629BPQ
CSB-EP386629BPQ
CSB-BP386629BPQ
CSB-MP386629BPQ
CSB-EP386629BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP386630BPQ
CSB-EP386630BPQ
CSB-BP386630BPQ
CSB-MP386630BPQ
CSB-EP386630BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP386631BPQ
CSB-EP386631BPQ
CSB-BP386631BPQ
CSB-MP386631BPQ
CSB-EP386631BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Sec-independent protein translocase protein TatA (tatA), partial CSB-YP386632BPQ
CSB-EP386632BPQ
CSB-BP386632BPQ
CSB-MP386632BPQ
CSB-EP386632BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP phosphoribosyltransferase (hisG) CSB-YP386633BPQ
CSB-EP386633BPQ
CSB-BP386633BPQ
CSB-MP386633BPQ
CSB-EP386633BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP386634BPQ
CSB-EP386634BPQ
CSB-BP386634BPQ
CSB-MP386634BPQ
CSB-EP386634BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphopantetheine adenylyltransferase (coaD) CSB-YP386635BPQ
CSB-EP386635BPQ
CSB-BP386635BPQ
CSB-MP386635BPQ
CSB-EP386635BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L25 (rplY) CSB-YP386636BPQ
CSB-EP386636BPQ
CSB-BP386636BPQ
CSB-MP386636BPQ
CSB-EP386636BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Carboxylate-amine ligase BMA10247_2975 (BMA10247_2975) CSB-YP386637BPQ
CSB-EP386637BPQ
CSB-BP386637BPQ
CSB-MP386637BPQ
CSB-EP386637BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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