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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia pseudomallei 50S ribosomal protein L36 (rpmJ) CSB-YP388019BPU
CSB-EP388019BPU
CSB-BP388019BPU
CSB-MP388019BPU
CSB-EP388019BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S5 (rpsE) CSB-YP388020BPU
CSB-EP388020BPU
CSB-BP388020BPU
CSB-MP388020BPU
CSB-EP388020BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L14 (rplN) CSB-YP388021BPU
CSB-EP388021BPU
CSB-BP388021BPU
CSB-MP388021BPU
CSB-EP388021BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S3 (rpsC) CSB-YP388022BPU
CSB-EP388022BPU
CSB-BP388022BPU
CSB-MP388022BPU
CSB-EP388022BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L4 (rplD) CSB-YP388023BPU
CSB-EP388023BPU
CSB-BP388023BPU
CSB-MP388023BPU
CSB-EP388023BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP388024BPU
CSB-EP388024BPU
CSB-BP388024BPU
CSB-MP388024BPU
CSB-EP388024BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L11 (rplK) CSB-YP388025BPU
CSB-EP388025BPU
CSB-BP388025BPU
CSB-MP388025BPU
CSB-EP388025BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP synthase gamma chain (atpG) CSB-YP388026BPU
CSB-EP388026BPU
CSB-BP388026BPU
CSB-MP388026BPU
CSB-EP388026BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable L-aspartate dehydrogenase (nadX) CSB-YP388027BPU
CSB-EP388027BPU
CSB-BP388027BPU
CSB-MP388027BPU
CSB-EP388027BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei PKHD-type hydroxylase BURPS668_A1690 (BURPS668_A1690) CSB-YP388028BPU
CSB-EP388028BPU
CSB-BP388028BPU
CSB-MP388028BPU
CSB-EP388028BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei HTH-type transcriptional regulator BetI (betI) CSB-YP388029BPU
CSB-EP388029BPU
CSB-BP388029BPU
CSB-MP388029BPU
CSB-EP388029BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Guanine nucleotide exchange factor BopE (bopE) CSB-YP388030BPU
CSB-EP388030BPU
CSB-BP388030BPU
CSB-MP388030BPU
CSB-EP388030BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Translocator protein BipB (bipB), partial CSB-YP388031BPU1
CSB-EP388031BPU1
CSB-BP388031BPU1
CSB-MP388031BPU1
CSB-EP388031BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP388032BPU
CSB-EP388032BPU
CSB-BP388032BPU
CSB-MP388032BPU
CSB-EP388032BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP synthase subunit beta 2 (atpD2), partial CSB-YP388033BPU
CSB-EP388033BPU
CSB-BP388033BPU
CSB-MP388033BPU
CSB-EP388033BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable D-serine dehydratase (dsdA) CSB-YP388034BPU
CSB-EP388034BPU
CSB-BP388034BPU
CSB-MP388034BPU
CSB-EP388034BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0391 membrane protein BURPS668_A3117 (BURPS668_A3117), partial CSB-YP388035BPU
CSB-EP388035BPU
CSB-BP388035BPU
CSB-MP388035BPU
CSB-EP388035BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L34 (rpmH) CSB-YP388036BPT
CSB-EP388036BPT
CSB-BP388036BPT
CSB-MP388036BPT
CSB-EP388036BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Membrane protein insertase YidC (yidC), partial CSB-YP388037BPT1
CSB-EP388037BPT1
CSB-BP388037BPT1
CSB-MP388037BPT1
CSB-EP388037BPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP388038BPT
CSB-EP388038BPT
CSB-BP388038BPT
CSB-MP388038BPT
CSB-EP388038BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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