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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP388420RIJ
CSB-EP388420RIJ
CSB-BP388420RIJ
CSB-MP388420RIJ
CSB-EP388420RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Proline--tRNA ligase (proS) CSB-YP388421RIJ
CSB-EP388421RIJ
CSB-BP388421RIJ
CSB-MP388421RIJ
CSB-EP388421RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Coenzyme PQQ synthesis protein A (pqqA) CSB-YP388422RIJ
CSB-EP388422RIJ
CSB-BP388422RIJ
CSB-MP388422RIJ
CSB-EP388422RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Peptidyl-tRNA hydrolase (pth) CSB-YP388423RIJ
CSB-EP388423RIJ
CSB-BP388423RIJ
CSB-MP388423RIJ
CSB-EP388423RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L25 (rplY) CSB-YP388424RIJ
CSB-EP388424RIJ
CSB-BP388424RIJ
CSB-MP388424RIJ
CSB-EP388424RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP388425RIJ
CSB-EP388425RIJ
CSB-BP388425RIJ
CSB-MP388425RIJ
CSB-EP388425RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Chromosome partition protein Smc (smc), partial CSB-YP388426RIJ
CSB-EP388426RIJ
CSB-BP388426RIJ
CSB-MP388426RIJ
CSB-EP388426RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP388427RIJ
CSB-EP388427RIJ
CSB-BP388427RIJ
CSB-MP388427RIJ
CSB-EP388427RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0301 protein Rsph17029_2659 (Rsph17029_2659) CSB-YP388428RIJ
CSB-EP388428RIJ
CSB-BP388428RIJ
CSB-MP388428RIJ
CSB-EP388428RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S16 (rpsP) CSB-YP388429RIJ
CSB-EP388429RIJ
CSB-BP388429RIJ
CSB-MP388429RIJ
CSB-EP388429RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Putative membrane protein insertion efficiency factor (Rsph17029_2722) CSB-YP388430RIJ
CSB-EP388430RIJ
CSB-BP388430RIJ
CSB-MP388430RIJ
CSB-EP388430RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Membrane protein insertase YidC (yidC), partial CSB-YP388431RIJ1
CSB-EP388431RIJ1
CSB-BP388431RIJ1
CSB-MP388431RIJ1
CSB-EP388431RIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosome-binding factor A (rbfA) CSB-YP388432RIJ
CSB-EP388432RIJ
CSB-BP388432RIJ
CSB-MP388432RIJ
CSB-EP388432RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP388433RIJ
CSB-EP388433RIJ
CSB-BP388433RIJ
CSB-MP388433RIJ
CSB-EP388433RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP388434RIJ
CSB-EP388434RIJ
CSB-BP388434RIJ
CSB-MP388434RIJ
CSB-EP388434RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribonuclease PH (rph) CSB-YP388435RIJ
CSB-EP388435RIJ
CSB-BP388435RIJ
CSB-MP388435RIJ
CSB-EP388435RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA modification GTPase MnmE (mnmE) CSB-YP388436RIJ
CSB-EP388436RIJ
CSB-BP388436RIJ
CSB-MP388436RIJ
CSB-EP388436RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP388437RIJ
CSB-EP388437RIJ
CSB-BP388437RIJ
CSB-MP388437RIJ
CSB-EP388437RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-dehydroquinate dehydratase (aroQ) CSB-YP388438RIJ
CSB-EP388438RIJ
CSB-BP388438RIJ
CSB-MP388438RIJ
CSB-EP388438RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Integration host factor subunit beta (ihfB) CSB-YP388439RIJ
CSB-EP388439RIJ
CSB-BP388439RIJ
CSB-MP388439RIJ
CSB-EP388439RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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