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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acinetobacter baumannii Phosphoglucosamine mutase (glmM) CSB-YP386578AUD
CSB-EP386578AUD
CSB-BP386578AUD
CSB-MP386578AUD
CSB-EP386578AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP386579AUD
CSB-EP386579AUD
CSB-BP386579AUD
CSB-MP386579AUD
CSB-EP386579AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Disulfide bond formation protein B (dsbB), partial CSB-YP386580AUD1
CSB-EP386580AUD1
CSB-BP386580AUD1
CSB-MP386580AUD1
CSB-EP386580AUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP386581BPQ
CSB-EP386581BPQ
CSB-BP386581BPQ
CSB-MP386581BPQ
CSB-EP386581BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Tryptophan synthase alpha chain (trpA) CSB-YP386582BPQ
CSB-EP386582BPQ
CSB-BP386582BPQ
CSB-MP386582BPQ
CSB-EP386582BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Effector protein BipC (bipC) CSB-YP386583BPQ
CSB-EP386583BPQ
CSB-BP386583BPQ
CSB-MP386583BPQ
CSB-EP386583BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP386584BPQ
CSB-EP386584BPQ
CSB-BP386584BPQ
CSB-MP386584BPQ
CSB-EP386584BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Betaine aldehyde dehydrogenase (betB) CSB-YP386585BPQ
CSB-EP386585BPQ
CSB-BP386585BPQ
CSB-MP386585BPQ
CSB-EP386585BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Acylphosphatase (acyP) CSB-YP386586BPQ
CSB-EP386586BPQ
CSB-BP386586BPQ
CSB-MP386586BPQ
CSB-EP386586BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0234 protein BMA10247_0122 (BMA10247_0122) CSB-YP386587BPQ
CSB-EP386587BPQ
CSB-BP386587BPQ
CSB-MP386587BPQ
CSB-EP386587BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP386588BPQ
CSB-EP386588BPQ
CSB-BP386588BPQ
CSB-MP386588BPQ
CSB-EP386588BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP386589BPQ
CSB-EP386589BPQ
CSB-BP386589BPQ
CSB-MP386589BPQ
CSB-EP386589BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Methylglyoxal synthase (mgsA) CSB-YP386590BPQ
CSB-EP386590BPQ
CSB-BP386590BPQ
CSB-MP386590BPQ
CSB-EP386590BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP386591BPQ
CSB-EP386591BPQ
CSB-BP386591BPQ
CSB-MP386591BPQ
CSB-EP386591BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit B 1 (nuoB1) CSB-YP386592BPQ
CSB-EP386592BPQ
CSB-BP386592BPQ
CSB-MP386592BPQ
CSB-EP386592BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP386593BPQ
CSB-EP386593BPQ
CSB-BP386593BPQ
CSB-MP386593BPQ
CSB-EP386593BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0060 membrane protein BMA10247_0554 (BMA10247_0554), partial CSB-YP386594BPQ1
CSB-EP386594BPQ1
CSB-BP386594BPQ1
CSB-MP386594BPQ1
CSB-EP386594BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Alanine--tRNA ligase (alaS), partial CSB-YP386595BPQ
CSB-EP386595BPQ
CSB-BP386595BPQ
CSB-MP386595BPQ
CSB-EP386595BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydroorotate dehydrogenase (quinone) CSB-YP386596BPQ
CSB-EP386596BPQ
CSB-BP386596BPQ
CSB-MP386596BPQ
CSB-EP386596BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei LexA repressor (lexA) CSB-YP386597BPQ
CSB-EP386597BPQ
CSB-BP386597BPQ
CSB-MP386597BPQ
CSB-EP386597BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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