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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP386878BPT
CSB-EP386878BPT
CSB-BP386878BPT
CSB-MP386878BPT
CSB-EP386878BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Bifunctional protein GlmU (glmU) CSB-YP386879BPT
CSB-EP386879BPT
CSB-BP386879BPT
CSB-MP386879BPT
CSB-EP386879BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 8-amino-7-oxononanoate synthase (bioF) CSB-YP386880BPT
CSB-EP386880BPT
CSB-BP386880BPT
CSB-MP386880BPT
CSB-EP386880BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Octanoyltransferase (lipB) CSB-YP386881BPT
CSB-EP386881BPT
CSB-BP386881BPT
CSB-MP386881BPT
CSB-EP386881BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei C4-dicarboxylate transport protein (dctA), partial CSB-YP386882BPT
CSB-EP386882BPT
CSB-BP386882BPT
CSB-MP386882BPT
CSB-EP386882BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Peptidyl-tRNA hydrolase (pth) CSB-YP386883BPT
CSB-EP386883BPT
CSB-BP386883BPT
CSB-MP386883BPT
CSB-EP386883BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP386884BPT
CSB-EP386884BPT
CSB-BP386884BPT
CSB-MP386884BPT
CSB-EP386884BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP386885BPT1
CSB-EP386885BPT1
CSB-BP386885BPT1
CSB-MP386885BPT1
CSB-EP386885BPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Kynurenine formamidase (kynB) CSB-YP386886BPT
CSB-EP386886BPT
CSB-BP386886BPT
CSB-MP386886BPT
CSB-EP386886BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S20 (rpsT) CSB-YP386887BPT
CSB-EP386887BPT
CSB-BP386887BPT
CSB-MP386887BPT
CSB-EP386887BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP386888BPT
CSB-EP386888BPT
CSB-BP386888BPT
CSB-MP386888BPT
CSB-EP386888BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP386889BPT
CSB-EP386889BPT
CSB-BP386889BPT
CSB-MP386889BPT
CSB-EP386889BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L28 (rpmB) CSB-YP386890BPT
CSB-EP386890BPT
CSB-BP386890BPT
CSB-MP386890BPT
CSB-EP386890BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable cytosol aminopeptidase (pepA), partial CSB-YP386891BPT
CSB-EP386891BPT
CSB-BP386891BPT
CSB-MP386891BPT
CSB-EP386891BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Pantothenate synthetase (panC) CSB-YP386892BPT
CSB-EP386892BPT
CSB-BP386892BPT
CSB-MP386892BPT
CSB-EP386892BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP386893BPT
CSB-EP386893BPT
CSB-BP386893BPT
CSB-MP386893BPT
CSB-EP386893BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Uracil phosphoribosyltransferase (upp) CSB-YP386894BPT
CSB-EP386894BPT
CSB-BP386894BPT
CSB-MP386894BPT
CSB-EP386894BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP386895BPT1
CSB-EP386895BPT1
CSB-BP386895BPT1
CSB-MP386895BPT1
CSB-EP386895BPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP386896BPT
CSB-EP386896BPT
CSB-BP386896BPT
CSB-MP386896BPT
CSB-EP386896BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP386897BPT
CSB-EP386897BPT
CSB-BP386897BPT
CSB-MP386897BPT
CSB-EP386897BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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