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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP386958PZE
CSB-EP386958PZE
CSB-BP386958PZE
CSB-MP386958PZE
CSB-EP386958PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit H CSB-YP386959PZE
CSB-EP386959PZE
CSB-BP386959PZE
CSB-MP386959PZE
CSB-EP386959PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP386960PZE
CSB-EP386960PZE
CSB-BP386960PZE
CSB-MP386960PZE
CSB-EP386960PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L11 (rplK) CSB-YP386961PZE
CSB-EP386961PZE
CSB-BP386961PZE
CSB-MP386961PZE
CSB-EP386961PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartate carbamoyltransferase (pyrB) CSB-YP386962PZE
CSB-EP386962PZE
CSB-BP386962PZE
CSB-MP386962PZE
CSB-EP386962PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Isoleucine--tRNA ligase (ileS), partial CSB-YP386963PZE
CSB-EP386963PZE
CSB-BP386963PZE
CSB-MP386963PZE
CSB-EP386963PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein psbN (psbN), partial CSB-YP386964PZE
CSB-EP386964PZE
CSB-BP386964PZE
CSB-MP386964PZE
CSB-EP386964PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP386965PZE
CSB-EP386965PZE
CSB-BP386965PZE
CSB-MP386965PZE
CSB-EP386965PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein K (psbK) CSB-YP386966PZE
CSB-EP386966PZE
CSB-BP386966PZE
CSB-MP386966PZE
CSB-EP386966PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit 3, partial CSB-YP386967PZE1
CSB-EP386967PZE1
CSB-BP386967PZE1
CSB-MP386967PZE1
CSB-EP386967PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein J (psbJ), partial CSB-YP386968PZE1
CSB-EP386968PZE1
CSB-BP386968PZE1
CSB-MP386968PZE1
CSB-EP386968PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP386969PZE1
CSB-EP386969PZE1
CSB-BP386969PZE1
CSB-MP386969PZE1
CSB-EP386969PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus GTPase Der (der) CSB-YP386970PZE
CSB-EP386970PZE
CSB-BP386970PZE
CSB-MP386970PZE
CSB-EP386970PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP386971PZE
CSB-EP386971PZE
CSB-BP386971PZE
CSB-MP386971PZE
CSB-EP386971PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable malate:quinone oxidoreductase (mqo) CSB-YP386972PZE
CSB-EP386972PZE
CSB-BP386972PZE
CSB-MP386972PZE
CSB-EP386972PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP386973PZE
CSB-EP386973PZE
CSB-BP386973PZE
CSB-MP386973PZE
CSB-EP386973PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP386974PZE1
CSB-EP386974PZE1
CSB-BP386974PZE1
CSB-MP386974PZE1
CSB-EP386974PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP386975PZE
CSB-EP386975PZE
CSB-BP386975PZE
CSB-MP386975PZE
CSB-EP386975PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adenylosuccinate synthetase (purA) CSB-YP386976PZE
CSB-EP386976PZE
CSB-BP386976PZE
CSB-MP386976PZE
CSB-EP386976PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP386977PZE
CSB-EP386977PZE
CSB-BP386977PZE
CSB-MP386977PZE
CSB-EP386977PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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