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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia pseudomallei 30S ribosomal protein S13 (rpsM) CSB-YP386858BPU
CSB-EP386858BPU
CSB-BP386858BPU
CSB-MP386858BPU
CSB-EP386858BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L30 (rpmD) CSB-YP386859BPU
CSB-EP386859BPU
CSB-BP386859BPU
CSB-MP386859BPU
CSB-EP386859BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L18 (rplR) CSB-YP386860BPU
CSB-EP386860BPU
CSB-BP386860BPU
CSB-MP386860BPU
CSB-EP386860BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L5 (rplE) CSB-YP386861BPU
CSB-EP386861BPU
CSB-BP386861BPU
CSB-MP386861BPU
CSB-EP386861BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L16 (rplP) CSB-YP386862BPU
CSB-EP386862BPU
CSB-BP386862BPU
CSB-MP386862BPU
CSB-EP386862BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L2 (rplB) CSB-YP386863BPU
CSB-EP386863BPU
CSB-BP386863BPU
CSB-MP386863BPU
CSB-EP386863BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S10 (rpsJ) CSB-YP386864BPU
CSB-EP386864BPU
CSB-BP386864BPU
CSB-MP386864BPU
CSB-EP386864BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP386865BPU
CSB-EP386865BPU
CSB-BP386865BPU
CSB-MP386865BPU
CSB-EP386865BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L7/L12 (rplL) CSB-YP386866BPU
CSB-EP386866BPU
CSB-BP386866BPU
CSB-MP386866BPU
CSB-EP386866BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP synthase subunit beta 1 (atpD1) CSB-YP386867BPU
CSB-EP386867BPU
CSB-BP386867BPU
CSB-MP386867BPU
CSB-EP386867BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP386868BPU
CSB-EP386868BPU
CSB-BP386868BPU
CSB-MP386868BPU
CSB-EP386868BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 60 kDa chaperonin 2 (groL2), partial CSB-YP386869BPU
CSB-EP386869BPU
CSB-BP386869BPU
CSB-MP386869BPU
CSB-EP386869BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0502 protein BURPS668_A1958 (BURPS668_A1958) CSB-YP386870BPU
CSB-EP386870BPU
CSB-BP386870BPU
CSB-MP386870BPU
CSB-EP386870BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD) CSB-YP386871BPU
CSB-EP386871BPU
CSB-BP386871BPU
CSB-MP386871BPU
CSB-EP386871BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP386872BPU
CSB-EP386872BPU
CSB-BP386872BPU
CSB-MP386872BPU
CSB-EP386872BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP386873BPU
CSB-EP386873BPU
CSB-BP386873BPU
CSB-MP386873BPU
CSB-EP386873BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP386874BPU
CSB-EP386874BPU
CSB-BP386874BPU
CSB-MP386874BPU
CSB-EP386874BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP386875BPT
CSB-EP386875BPT
CSB-BP386875BPT
CSB-MP386875BPT
CSB-EP386875BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribonuclease P protein component (rnpA) CSB-YP386876BPT
CSB-EP386876BPT
CSB-BP386876BPT
CSB-MP386876BPT
CSB-EP386876BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP386877BPT
CSB-EP386877BPT
CSB-BP386877BPT
CSB-MP386877BPT
CSB-EP386877BPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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