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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella loihica Leucine--tRNA ligase (leuS), partial CSB-YP388681STO
CSB-EP388681STO
CSB-BP388681STO
CSB-MP388681STO
CSB-EP388681STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S6 (rpsF) CSB-YP388682STO
CSB-EP388682STO
CSB-BP388682STO
CSB-MP388682STO
CSB-EP388682STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S9 (rpsI) CSB-YP388683STO
CSB-EP388683STO
CSB-BP388683STO
CSB-MP388683STO
CSB-EP388683STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP388684STO
CSB-EP388684STO
CSB-BP388684STO
CSB-MP388684STO
CSB-EP388684STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Sec-independent protein translocase protein TatA (tatA), partial CSB-YP388685STO
CSB-EP388685STO
CSB-BP388685STO
CSB-MP388685STO
CSB-EP388685STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP388686STO
CSB-EP388686STO
CSB-BP388686STO
CSB-MP388686STO
CSB-EP388686STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP388687STO
CSB-EP388687STO
CSB-BP388687STO
CSB-MP388687STO
CSB-EP388687STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP388688STO
CSB-EP388688STO
CSB-BP388688STO
CSB-MP388688STO
CSB-EP388688STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP388689STO
CSB-EP388689STO
CSB-BP388689STO
CSB-MP388689STO
CSB-EP388689STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP388690STO
CSB-EP388690STO
CSB-BP388690STO
CSB-MP388690STO
CSB-EP388690STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP388691STO
CSB-EP388691STO
CSB-BP388691STO
CSB-MP388691STO
CSB-EP388691STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP388692STO
CSB-EP388692STO
CSB-BP388692STO
CSB-MP388692STO
CSB-EP388692STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP388693STO
CSB-EP388693STO
CSB-BP388693STO
CSB-MP388693STO
CSB-EP388693STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP388694STO
CSB-EP388694STO
CSB-BP388694STO
CSB-MP388694STO
CSB-EP388694STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP388695STO
CSB-EP388695STO
CSB-BP388695STO
CSB-MP388695STO
CSB-EP388695STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ATP synthase gamma chain (atpG) CSB-YP388696STO
CSB-EP388696STO
CSB-BP388696STO
CSB-MP388696STO
CSB-EP388696STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP388697STO
CSB-EP388697STO
CSB-BP388697STO
CSB-MP388697STO
CSB-EP388697STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Putative membrane protein insertion efficiency factor (Shew_3867) CSB-YP388698STO
CSB-EP388698STO
CSB-BP388698STO
CSB-MP388698STO
CSB-EP388698STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma benhamiae Sulfite efflux pump SSU1 (SSU1), partial CSB-YP388699DON
CSB-EP388699DON
CSB-BP388699DON
CSB-MP388699DON
CSB-EP388699DON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant KI polyomavirus Minor capsid protein VP2 CSB-YP388700KAV
CSB-EP388700KAV
CSB-BP388700KAV
CSB-MP388700KAV
CSB-EP388700KAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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