Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfotomaculum reducens Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP391262DHX
CSB-EP391262DHX
CSB-BP391262DHX
CSB-MP391262DHX
CSB-EP391262DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Adenylosuccinate synthetase (purA) CSB-YP391263DHX
CSB-EP391263DHX
CSB-BP391263DHX
CSB-MP391263DHX
CSB-EP391263DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP391264DHX
CSB-EP391264DHX
CSB-BP391264DHX
CSB-MP391264DHX
CSB-EP391264DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP391265BPV
CSB-EP391265BPV
CSB-BP391265BPV
CSB-MP391265BPV
CSB-EP391265BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP391266BPV
CSB-EP391266BPV
CSB-BP391266BPV
CSB-MP391266BPV
CSB-EP391266BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L11 (rplK) CSB-YP391267BPV
CSB-EP391267BPV
CSB-BP391267BPV
CSB-MP391267BPV
CSB-EP391267BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S12 (rpsL) CSB-YP391268BPV
CSB-EP391268BPV
CSB-BP391268BPV
CSB-MP391268BPV
CSB-EP391268BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S10 (rpsJ) CSB-YP391269BPV
CSB-EP391269BPV
CSB-BP391269BPV
CSB-MP391269BPV
CSB-EP391269BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L2 (rplB) CSB-YP391270BPV
CSB-EP391270BPV
CSB-BP391270BPV
CSB-MP391270BPV
CSB-EP391270BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S3 (rpsC) CSB-YP391271BPV
CSB-EP391271BPV
CSB-BP391271BPV
CSB-MP391271BPV
CSB-EP391271BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S17 (rpsQ) CSB-YP391272BPV
CSB-EP391272BPV
CSB-BP391272BPV
CSB-MP391272BPV
CSB-EP391272BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L5 (rplE) CSB-YP391273BPV
CSB-EP391273BPV
CSB-BP391273BPV
CSB-MP391273BPV
CSB-EP391273BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L30 (rpmD) CSB-YP391274BPV
CSB-EP391274BPV
CSB-BP391274BPV
CSB-MP391274BPV
CSB-EP391274BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L36 (rpmJ) CSB-YP391275BPV
CSB-EP391275BPV
CSB-BP391275BPV
CSB-MP391275BPV
CSB-EP391275BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S4 (rpsD) CSB-YP391276BPV
CSB-EP391276BPV
CSB-BP391276BPV
CSB-MP391276BPV
CSB-EP391276BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Probable GTP-binding protein EngB (engB) CSB-YP391277BPV
CSB-EP391277BPV
CSB-BP391277BPV
CSB-MP391277BPV
CSB-EP391277BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 3-dehydroquinate synthase (aroB) CSB-YP391278BPV
CSB-EP391278BPV
CSB-BP391278BPV
CSB-MP391278BPV
CSB-EP391278BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP391279BPV
CSB-EP391279BPV
CSB-BP391279BPV
CSB-MP391279BPV
CSB-EP391279BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Glutamyl-tRNA reductase (hemA) CSB-YP391280BPV
CSB-EP391280BPV
CSB-BP391280BPV
CSB-MP391280BPV
CSB-EP391280BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP391281BPV
CSB-EP391281BPV
CSB-BP391281BPV
CSB-MP391281BPV
CSB-EP391281BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>