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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polynucleobacter sp. Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP391862PYU
CSB-EP391862PYU
CSB-BP391862PYU
CSB-MP391862PYU
CSB-EP391862PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP391863PYU
CSB-EP391863PYU
CSB-BP391863PYU
CSB-MP391863PYU
CSB-EP391863PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. UPF0173 metal-dependent hydrolase Pnuc_1524 (Pnuc_1524) CSB-YP391864PYU
CSB-EP391864PYU
CSB-BP391864PYU
CSB-MP391864PYU
CSB-EP391864PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S21 (rpsU) CSB-YP391865PYU
CSB-EP391865PYU
CSB-BP391865PYU
CSB-MP391865PYU
CSB-EP391865PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L33 (rpmG) CSB-YP391866PYU
CSB-EP391866PYU
CSB-BP391866PYU
CSB-MP391866PYU
CSB-EP391866PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP391867PYU
CSB-EP391867PYU
CSB-BP391867PYU
CSB-MP391867PYU
CSB-EP391867PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP391868PYU
CSB-EP391868PYU
CSB-BP391868PYU
CSB-MP391868PYU
CSB-EP391868PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Probable rRNA maturation factor (Pnuc_1860) CSB-YP391869PYU
CSB-EP391869PYU
CSB-BP391869PYU
CSB-MP391869PYU
CSB-EP391869PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP391870PYU
CSB-EP391870PYU
CSB-BP391870PYU
CSB-MP391870PYU
CSB-EP391870PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP391871PYU
CSB-EP391871PYU
CSB-BP391871PYU
CSB-MP391871PYU
CSB-EP391871PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Peptidyl-tRNA hydrolase (pth) CSB-YP391872PYU
CSB-EP391872PYU
CSB-BP391872PYU
CSB-MP391872PYU
CSB-EP391872PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Octanoyltransferase (lipB) CSB-YP391873PYU
CSB-EP391873PYU
CSB-BP391873PYU
CSB-MP391873PYU
CSB-EP391873PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Orotate phosphoribosyltransferase (pyrE) CSB-YP391874PYU
CSB-EP391874PYU
CSB-BP391874PYU
CSB-MP391874PYU
CSB-EP391874PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. C4-dicarboxylate transport protein (dctA), partial CSB-YP391875PYU
CSB-EP391875PYU
CSB-BP391875PYU
CSB-MP391875PYU
CSB-EP391875PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Membrane protein insertase YidC (yidC), partial CSB-YP391876PYU1
CSB-EP391876PYU1
CSB-BP391876PYU1
CSB-MP391876PYU1
CSB-EP391876PYU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Adenylosuccinate synthetase (purA) CSB-YP391877MOK
CSB-EP391877MOK
CSB-BP391877MOK
CSB-MP391877MOK
CSB-EP391877MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Protease HtpX homolog (htpX), partial CSB-YP391878MOK1
CSB-EP391878MOK1
CSB-BP391878MOK1
CSB-MP391878MOK1
CSB-EP391878MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L1 (rplA) CSB-YP391879MOK
CSB-EP391879MOK
CSB-BP391879MOK
CSB-MP391879MOK
CSB-EP391879MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L10 (rplJ) CSB-YP391880MOK
CSB-EP391880MOK
CSB-BP391880MOK
CSB-MP391880MOK
CSB-EP391880MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum DNA repair protein recO (recO) CSB-YP391881MOK
CSB-EP391881MOK
CSB-BP391881MOK
CSB-MP391881MOK
CSB-EP391881MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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