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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Burkholderia vietnamiensis UPF0246 protein Bcep1808_2308 (Bcep1808_2308) CSB-YP391322BPV
CSB-EP391322BPV
CSB-BP391322BPV
CSB-MP391322BPV
CSB-EP391322BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP391323BPV
CSB-EP391323BPV
CSB-BP391323BPV
CSB-MP391323BPV
CSB-EP391323BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP391324BPV1
CSB-EP391324BPV1
CSB-BP391324BPV1
CSB-MP391324BPV1
CSB-EP391324BPV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 2-isopropylmalate synthase (leuA), partial CSB-YP391325BPV
CSB-EP391325BPV
CSB-BP391325BPV
CSB-MP391325BPV
CSB-EP391325BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP391326BPV
CSB-EP391326BPV
CSB-BP391326BPV
CSB-MP391326BPV
CSB-EP391326BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP391327BPV
CSB-EP391327BPV
CSB-BP391327BPV
CSB-MP391327BPV
CSB-EP391327BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Pantothenate synthetase (panC) CSB-YP391328BPV
CSB-EP391328BPV
CSB-BP391328BPV
CSB-MP391328BPV
CSB-EP391328BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Probable cytosol aminopeptidase (pepA), partial CSB-YP391329BPV
CSB-EP391329BPV
CSB-BP391329BPV
CSB-MP391329BPV
CSB-EP391329BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP391330BPV
CSB-EP391330BPV
CSB-BP391330BPV
CSB-MP391330BPV
CSB-EP391330BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0434 protein Bcep1808_2639 (Bcep1808_2639) CSB-YP391331BPV
CSB-EP391331BPV
CSB-BP391331BPV
CSB-MP391331BPV
CSB-EP391331BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Kynurenine formamidase (kynB) CSB-YP391332BPV
CSB-EP391332BPV
CSB-BP391332BPV
CSB-MP391332BPV
CSB-EP391332BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Glycerol kinase (glpK) CSB-YP391333BPV
CSB-EP391333BPV
CSB-BP391333BPV
CSB-MP391333BPV
CSB-EP391333BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Aspartate--tRNA ligase (aspS), partial CSB-YP391334BPV
CSB-EP391334BPV
CSB-BP391334BPV
CSB-MP391334BPV
CSB-EP391334BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis HPr kinase/phosphorylase (hprK) CSB-YP391335BPV
CSB-EP391335BPV
CSB-BP391335BPV
CSB-MP391335BPV
CSB-EP391335BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phosphopantetheine adenylyltransferase (coaD) CSB-YP391336BPV
CSB-EP391336BPV
CSB-BP391336BPV
CSB-MP391336BPV
CSB-EP391336BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis C4-dicarboxylate transport protein (dctA), partial CSB-YP391337BPV
CSB-EP391337BPV
CSB-BP391337BPV
CSB-MP391337BPV
CSB-EP391337BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP391338BPV
CSB-EP391338BPV
CSB-BP391338BPV
CSB-MP391338BPV
CSB-EP391338BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP391339BPV
CSB-EP391339BPV
CSB-BP391339BPV
CSB-MP391339BPV
CSB-EP391339BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Flagellar L-ring protein (flgH) CSB-YP391340BPV
CSB-EP391340BPV
CSB-BP391340BPV
CSB-MP391340BPV
CSB-EP391340BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis S-adenosylmethionine synthase (metK) CSB-YP391341BPV
CSB-EP391341BPV
CSB-BP391341BPV
CSB-MP391341BPV
CSB-EP391341BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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