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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aeromonas salmonicida Cytidylate kinase (cmk) CSB-YP392662AUI
CSB-EP392662AUI
CSB-BP392662AUI
CSB-MP392662AUI
CSB-EP392662AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Adenylate kinase (adk) CSB-YP392663AUI
CSB-EP392663AUI
CSB-BP392663AUI
CSB-MP392663AUI
CSB-EP392663AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP392664AUI
CSB-EP392664AUI
CSB-BP392664AUI
CSB-MP392664AUI
CSB-EP392664AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L35 (rpmI) CSB-YP392665AUI
CSB-EP392665AUI
CSB-BP392665AUI
CSB-MP392665AUI
CSB-EP392665AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Protein proQ homolog (proQ) CSB-YP392666AUI
CSB-EP392666AUI
CSB-BP392666AUI
CSB-MP392666AUI
CSB-EP392666AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP392667AUI
CSB-EP392667AUI
CSB-BP392667AUI
CSB-MP392667AUI
CSB-EP392667AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Uridine kinase (udk) CSB-YP392668AUI
CSB-EP392668AUI
CSB-BP392668AUI
CSB-MP392668AUI
CSB-EP392668AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP392669AUI
CSB-EP392669AUI
CSB-BP392669AUI
CSB-MP392669AUI
CSB-EP392669AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida ATP phosphoribosyltransferase (hisG) CSB-YP392670AUI
CSB-EP392670AUI
CSB-BP392670AUI
CSB-MP392670AUI
CSB-EP392670AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP392671AUI
CSB-EP392671AUI
CSB-BP392671AUI
CSB-MP392671AUI
CSB-EP392671AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0345 protein ASA_2162 (ASA_2162) CSB-YP392672AUI
CSB-EP392672AUI
CSB-BP392672AUI
CSB-MP392672AUI
CSB-EP392672AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida FMN-dependent NADH-azoreductase (azoR) CSB-YP392673AUI
CSB-EP392673AUI
CSB-BP392673AUI
CSB-MP392673AUI
CSB-EP392673AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Serine/threonine transporter sstT (sstT), partial CSB-YP392674AUI
CSB-EP392674AUI
CSB-BP392674AUI
CSB-MP392674AUI
CSB-EP392674AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Tryptophanase (tnaA) CSB-YP392675AUI
CSB-EP392675AUI
CSB-BP392675AUI
CSB-MP392675AUI
CSB-EP392675AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L36 1 (rpmJ1) CSB-YP392676AUI
CSB-EP392676AUI
CSB-BP392676AUI
CSB-MP392676AUI
CSB-EP392676AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Putative pterin-4-alpha-carbinolamine dehydratase (ASA_2419) CSB-YP392677AUI
CSB-EP392677AUI
CSB-BP392677AUI
CSB-MP392677AUI
CSB-EP392677AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Translation initiation factor IF-1 (infA) CSB-YP392678AUI
CSB-EP392678AUI
CSB-BP392678AUI
CSB-MP392678AUI
CSB-EP392678AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Electron transport complex protein RnfB (rnfB) CSB-YP392679AUI
CSB-EP392679AUI
CSB-BP392679AUI
CSB-MP392679AUI
CSB-EP392679AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Putative phosphotransferase ASA_2545 (ASA_2545) CSB-YP392680AUI
CSB-EP392680AUI
CSB-BP392680AUI
CSB-MP392680AUI
CSB-EP392680AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Histidine--tRNA ligase (hisS) CSB-YP392681AUI
CSB-EP392681AUI
CSB-BP392681AUI
CSB-MP392681AUI
CSB-EP392681AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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