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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Crucihimalaya wallichii ATP synthase subunit beta, chloroplastic (atpB) CSB-YP391542CXN
CSB-EP391542CXN
CSB-BP391542CXN
CSB-MP391542CXN
CSB-EP391542CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP391543CXN1
CSB-EP391543CXN1
CSB-BP391543CXN1
CSB-MP391543CXN1
CSB-EP391543CXN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii Photosystem II reaction center protein J (psbJ), partial CSB-YP391544CXN
CSB-EP391544CXN
CSB-BP391544CXN
CSB-MP391544CXN
CSB-EP391544CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP391545CXN
CSB-EP391545CXN
CSB-BP391545CXN
CSB-MP391545CXN
CSB-EP391545CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP391546CXN
CSB-EP391546CXN
CSB-BP391546CXN
CSB-MP391546CXN
CSB-EP391546CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP391547CXN
CSB-EP391547CXN
CSB-BP391547CXN
CSB-MP391547CXN
CSB-EP391547CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP391548CXN
CSB-EP391548CXN
CSB-BP391548CXN
CSB-MP391548CXN
CSB-EP391548CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii Putative uncharacterized protein ycf15 (ycf15-A) CSB-YP391549CXN
CSB-EP391549CXN
CSB-BP391549CXN
CSB-MP391549CXN
CSB-EP391549CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP391550CXN
CSB-EP391550CXN
CSB-BP391550CXN
CSB-MP391550CXN
CSB-EP391550CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP391551CXN1
CSB-EP391551CXN1
CSB-BP391551CXN1
CSB-MP391551CXN1
CSB-EP391551CXN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii NAD (P)H-quinone oxidoreductase subunit 1, chloroplastic, partial CSB-YP391552CXN
CSB-EP391552CXN
CSB-BP391552CXN
CSB-MP391552CXN
CSB-EP391552CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP391553CXN
CSB-EP391553CXN
CSB-BP391553CXN
CSB-MP391553CXN
CSB-EP391553CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crucihimalaya wallichii Protein ycf2 B (ycf2-B), partial CSB-YP391554CXN
CSB-EP391554CXN
CSB-BP391554CXN
CSB-MP391554CXN
CSB-EP391554CXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Photosystem II reaction center protein I (psbI), partial CSB-YP391555DIE
CSB-EP391555DIE
CSB-BP391555DIE
CSB-MP391555DIE
CSB-EP391555DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP391556DIE1
CSB-EP391556DIE1
CSB-BP391556DIE1
CSB-MP391556DIE1
CSB-EP391556DIE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP391557DIE
CSB-EP391557DIE
CSB-BP391557DIE
CSB-MP391557DIE
CSB-EP391557DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP391558DIE
CSB-EP391558DIE
CSB-BP391558DIE
CSB-MP391558DIE
CSB-EP391558DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP391559DIE
CSB-EP391559DIE
CSB-BP391559DIE
CSB-MP391559DIE
CSB-EP391559DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Photosystem II reaction center protein J (psbJ), partial CSB-YP391560DIE
CSB-EP391560DIE
CSB-BP391560DIE
CSB-MP391560DIE
CSB-EP391560DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Draba nemorosa Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP391561DIE
CSB-EP391561DIE
CSB-BP391561DIE
CSB-MP391561DIE
CSB-EP391561DIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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