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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas salmonicida High frequency lysogenization protein HflD homolog (hflD) CSB-YP391742AUI
CSB-EP391742AUI
CSB-BP391742AUI
CSB-MP391742AUI
CSB-EP391742AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Tryptophan synthase alpha chain (trpA) CSB-YP391743AUI
CSB-EP391743AUI
CSB-BP391743AUI
CSB-MP391743AUI
CSB-EP391743AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0502 protein ASA_1460 (ASA_1460) CSB-YP391744AUI
CSB-EP391744AUI
CSB-BP391744AUI
CSB-MP391744AUI
CSB-EP391744AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP391745AUI
CSB-EP391745AUI
CSB-BP391745AUI
CSB-MP391745AUI
CSB-EP391745AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Dihydrodipicolinate reductase (dapB) CSB-YP391746AUI
CSB-EP391746AUI
CSB-BP391746AUI
CSB-MP391746AUI
CSB-EP391746AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP391747AUI
CSB-EP391747AUI
CSB-BP391747AUI
CSB-MP391747AUI
CSB-EP391747AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP391748AUI
CSB-EP391748AUI
CSB-BP391748AUI
CSB-MP391748AUI
CSB-EP391748AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Chaperone protein htpG (htpG), partial CSB-YP391749AUI
CSB-EP391749AUI
CSB-BP391749AUI
CSB-MP391749AUI
CSB-EP391749AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP391750AUI
CSB-EP391750AUI
CSB-BP391750AUI
CSB-MP391750AUI
CSB-EP391750AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP391751AUI
CSB-EP391751AUI
CSB-BP391751AUI
CSB-MP391751AUI
CSB-EP391751AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Threonine--tRNA ligase (thrS), partial CSB-YP391752AUI
CSB-EP391752AUI
CSB-BP391752AUI
CSB-MP391752AUI
CSB-EP391752AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L20 (rplT) CSB-YP391753AUI
CSB-EP391753AUI
CSB-BP391753AUI
CSB-MP391753AUI
CSB-EP391753AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP391754AUI
CSB-EP391754AUI
CSB-BP391754AUI
CSB-MP391754AUI
CSB-EP391754AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Dihydroorotate dehydrogenase (quinone) CSB-YP391755AUI
CSB-EP391755AUI
CSB-BP391755AUI
CSB-MP391755AUI
CSB-EP391755AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L32 (rpmF) CSB-YP391756AUI
CSB-EP391756AUI
CSB-BP391756AUI
CSB-MP391756AUI
CSB-EP391756AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Acylphosphatase (acyP) CSB-YP391757AUI
CSB-EP391757AUI
CSB-BP391757AUI
CSB-MP391757AUI
CSB-EP391757AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida tRNA 2-selenouridine synthase (selU) CSB-YP391758AUI
CSB-EP391758AUI
CSB-BP391758AUI
CSB-MP391758AUI
CSB-EP391758AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L25 (rplY) CSB-YP391759AUI
CSB-EP391759AUI
CSB-BP391759AUI
CSB-MP391759AUI
CSB-EP391759AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Histidinol-phosphate aminotransferase (hisC) CSB-YP391760AUI
CSB-EP391760AUI
CSB-BP391760AUI
CSB-MP391760AUI
CSB-EP391760AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0745 protein ASA_2166 (ASA_2166) CSB-YP391761AUI
CSB-EP391761AUI
CSB-BP391761AUI
CSB-MP391761AUI
CSB-EP391761AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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