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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Olimarabidopsis pumila Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP391482ODI1
CSB-EP391482ODI1
CSB-BP391482ODI1
CSB-MP391482ODI1
CSB-EP391482ODI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP391483ODI
CSB-EP391483ODI
CSB-BP391483ODI
CSB-MP391483ODI
CSB-EP391483ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP391484ODI
CSB-EP391484ODI
CSB-BP391484ODI
CSB-MP391484ODI
CSB-EP391484ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP391485ODI
CSB-EP391485ODI
CSB-BP391485ODI
CSB-MP391485ODI
CSB-EP391485ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila Protein ycf2 (ycf2-A), partial CSB-YP391486ODI
CSB-EP391486ODI
CSB-BP391486ODI
CSB-MP391486ODI
CSB-EP391486ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP391487ODI
CSB-EP391487ODI
CSB-BP391487ODI
CSB-MP391487ODI
CSB-EP391487ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP391488ODI
CSB-EP391488ODI
CSB-BP391488ODI
CSB-MP391488ODI
CSB-EP391488ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olimarabidopsis pumila Photosystem II reaction center protein J (psbJ), partial CSB-YP391489ODI
CSB-EP391489ODI
CSB-BP391489ODI
CSB-MP391489ODI
CSB-EP391489ODI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP391490AUX
CSB-EP391490AUX
CSB-BP391490AUX
CSB-MP391490AUX
CSB-EP391490AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP391491AUX
CSB-EP391491AUX
CSB-BP391491AUX
CSB-MP391491AUX
CSB-EP391491AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP391492AUX1
CSB-EP391492AUX1
CSB-BP391492AUX1
CSB-MP391492AUX1
CSB-EP391492AUX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP391493AUX
CSB-EP391493AUX
CSB-BP391493AUX
CSB-MP391493AUX
CSB-EP391493AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP391494AUX
CSB-EP391494AUX
CSB-BP391494AUX
CSB-MP391494AUX
CSB-EP391494AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta ATP synthase subunit beta, chloroplastic (atpB) CSB-YP391495AUX
CSB-EP391495AUX
CSB-BP391495AUX
CSB-MP391495AUX
CSB-EP391495AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta Chloroplast envelope membrane protein (cemA), partial CSB-YP391496AUX1
CSB-EP391496AUX1
CSB-BP391496AUX1
CSB-MP391496AUX1
CSB-EP391496AUX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta Photosystem II reaction center protein J (psbJ), partial CSB-YP391497AUX
CSB-EP391497AUX
CSB-BP391497AUX
CSB-MP391497AUX
CSB-EP391497AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP391498AUX
CSB-EP391498AUX
CSB-BP391498AUX
CSB-MP391498AUX
CSB-EP391498AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP391499AUX
CSB-EP391499AUX
CSB-BP391499AUX
CSB-MP391499AUX
CSB-EP391499AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP391500AUX
CSB-EP391500AUX
CSB-BP391500AUX
CSB-MP391500AUX
CSB-EP391500AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP391501AUX
CSB-EP391501AUX
CSB-BP391501AUX
CSB-MP391501AUX
CSB-EP391501AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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