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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabis hirsuta Protein ycf2 (ycf2-A), partial CSB-YP391502AUX
CSB-EP391502AUX
CSB-BP391502AUX
CSB-MP391502AUX
CSB-EP391502AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP391503AUX
CSB-EP391503AUX
CSB-BP391503AUX
CSB-MP391503AUX
CSB-EP391503AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabis hirsuta NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP391504AUX
CSB-EP391504AUX
CSB-BP391504AUX
CSB-MP391504AUX
CSB-EP391504AUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP391505BOQ1
CSB-EP391505BOQ1
CSB-BP391505BOQ1
CSB-MP391505BOQ1
CSB-EP391505BOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Photosystem II D2 protein (psbD), partial CSB-YP391506BOQ1
CSB-EP391506BOQ1
CSB-BP391506BOQ1
CSB-MP391506BOQ1
CSB-EP391506BOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP391507BOQ
CSB-EP391507BOQ
CSB-BP391507BOQ
CSB-MP391507BOQ
CSB-EP391507BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP391508BOQ
CSB-EP391508BOQ
CSB-BP391508BOQ
CSB-MP391508BOQ
CSB-EP391508BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD) CSB-YP391509BOQ
CSB-EP391509BOQ
CSB-BP391509BOQ
CSB-MP391509BOQ
CSB-EP391509BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Photosystem II reaction center protein J (psbJ), partial CSB-YP391510BOQ
CSB-EP391510BOQ
CSB-BP391510BOQ
CSB-MP391510BOQ
CSB-EP391510BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Photosystem I reaction center subunit IX (psaJ), partial CSB-YP391511BOQ
CSB-EP391511BOQ
CSB-BP391511BOQ
CSB-MP391511BOQ
CSB-EP391511BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Protein psbN (psbN), partial CSB-YP391512BOQ
CSB-EP391512BOQ
CSB-BP391512BOQ
CSB-MP391512BOQ
CSB-EP391512BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP391513BOQ
CSB-EP391513BOQ
CSB-BP391513BOQ
CSB-MP391513BOQ
CSB-EP391513BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP391514BOQ
CSB-EP391514BOQ
CSB-BP391514BOQ
CSB-MP391514BOQ
CSB-EP391514BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP391515BOQ
CSB-EP391515BOQ
CSB-BP391515BOQ
CSB-MP391515BOQ
CSB-EP391515BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP391516BOQ
CSB-EP391516BOQ
CSB-BP391516BOQ
CSB-MP391516BOQ
CSB-EP391516BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP391517BOQ1
CSB-EP391517BOQ1
CSB-BP391517BOQ1
CSB-MP391517BOQ1
CSB-EP391517BOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP391518BOQ1
CSB-EP391518BOQ1
CSB-BP391518BOQ1
CSB-MP391518BOQ1
CSB-EP391518BOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barbarea verna 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP391519BOQ
CSB-EP391519BOQ
CSB-BP391519BOQ
CSB-MP391519BOQ
CSB-EP391519BOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsella bursa-pastoris ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP391520CWI1
CSB-EP391520CWI1
CSB-BP391520CWI1
CSB-MP391520CWI1
CSB-EP391520CWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsella bursa-pastoris 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP391521CWI
CSB-EP391521CWI
CSB-BP391521CWI
CSB-MP391521CWI
CSB-EP391521CWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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