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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas salmonicida Maltoporin 2 (lamB2) CSB-YP391782AUI
CSB-EP391782AUI
CSB-BP391782AUI
CSB-MP391782AUI
CSB-EP391782AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP391783AUI1
CSB-EP391783AUI1
CSB-BP391783AUI1
CSB-MP391783AUI1
CSB-EP391783AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Hydroxylamine reductase (hcp), partial CSB-YP391784AUI
CSB-EP391784AUI
CSB-BP391784AUI
CSB-MP391784AUI
CSB-EP391784AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP391785AUI
CSB-EP391785AUI
CSB-BP391785AUI
CSB-MP391785AUI
CSB-EP391785AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L21 (rplU) CSB-YP391786AUI
CSB-EP391786AUI
CSB-BP391786AUI
CSB-MP391786AUI
CSB-EP391786AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 2-isopropylmalate synthase (leuA), partial CSB-YP391787AUI
CSB-EP391787AUI
CSB-BP391787AUI
CSB-MP391787AUI
CSB-EP391787AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP391788AUI
CSB-EP391788AUI
CSB-BP391788AUI
CSB-MP391788AUI
CSB-EP391788AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Multidrug resistance protein MdtH (mdtH), partial CSB-YP391789AUI
CSB-EP391789AUI
CSB-BP391789AUI
CSB-MP391789AUI
CSB-EP391789AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP391790AUI
CSB-EP391790AUI
CSB-BP391790AUI
CSB-MP391790AUI
CSB-EP391790AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 5'-nucleotidase surE (surE) CSB-YP391791AUI
CSB-EP391791AUI
CSB-BP391791AUI
CSB-MP391791AUI
CSB-EP391791AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP391792AUI
CSB-EP391792AUI
CSB-BP391792AUI
CSB-MP391792AUI
CSB-EP391792AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Siroheme synthase 2 (cysG2) CSB-YP391793AUI
CSB-EP391793AUI
CSB-BP391793AUI
CSB-MP391793AUI
CSB-EP391793AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP391794AUI
CSB-EP391794AUI
CSB-BP391794AUI
CSB-MP391794AUI
CSB-EP391794AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Deoxyribose-phosphate aldolase (deoC) CSB-YP391795AUI
CSB-EP391795AUI
CSB-BP391795AUI
CSB-MP391795AUI
CSB-EP391795AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP391796AUI
CSB-EP391796AUI
CSB-BP391796AUI
CSB-MP391796AUI
CSB-EP391796AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0178 protein ASA_3749 (ASA_3749) CSB-YP391797AUI
CSB-EP391797AUI
CSB-BP391797AUI
CSB-MP391797AUI
CSB-EP391797AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Thiazole synthase (thiG) CSB-YP391798AUI
CSB-EP391798AUI
CSB-BP391798AUI
CSB-MP391798AUI
CSB-EP391798AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L17 (rplQ) CSB-YP391799AUI
CSB-EP391799AUI
CSB-BP391799AUI
CSB-MP391799AUI
CSB-EP391799AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L36 2 (rpmJ2) CSB-YP391800AUI
CSB-EP391800AUI
CSB-BP391800AUI
CSB-MP391800AUI
CSB-EP391800AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S5 (rpsE) CSB-YP391801AUI
CSB-EP391801AUI
CSB-BP391801AUI
CSB-MP391801AUI
CSB-EP391801AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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