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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polynucleobacter sp. 50S ribosomal protein L2 (rplB) CSB-YP391822PYU
CSB-EP391822PYU
CSB-BP391822PYU
CSB-MP391822PYU
CSB-EP391822PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S3 (rpsC) CSB-YP391823PYU
CSB-EP391823PYU
CSB-BP391823PYU
CSB-MP391823PYU
CSB-EP391823PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L24 (rplX) CSB-YP391824PYU
CSB-EP391824PYU
CSB-BP391824PYU
CSB-MP391824PYU
CSB-EP391824PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S11 (rpsK) CSB-YP391825PYU
CSB-EP391825PYU
CSB-BP391825PYU
CSB-MP391825PYU
CSB-EP391825PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Probable GTP-binding protein EngB (engB) CSB-YP391826PYU
CSB-EP391826PYU
CSB-BP391826PYU
CSB-MP391826PYU
CSB-EP391826PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP391827PYU
CSB-EP391827PYU
CSB-BP391827PYU
CSB-MP391827PYU
CSB-EP391827PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Sec-independent protein translocase protein TatA (tatA), partial CSB-YP391828PYU
CSB-EP391828PYU
CSB-BP391828PYU
CSB-MP391828PYU
CSB-EP391828PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Biotin synthase (bioB) CSB-YP391829PYU
CSB-EP391829PYU
CSB-BP391829PYU
CSB-MP391829PYU
CSB-EP391829PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP391830PYU
CSB-EP391830PYU
CSB-BP391830PYU
CSB-MP391830PYU
CSB-EP391830PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L27 (rpmA) CSB-YP391831PYU
CSB-EP391831PYU
CSB-BP391831PYU
CSB-MP391831PYU
CSB-EP391831PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Leucine--tRNA ligase (leuS), partial CSB-YP391832PYU
CSB-EP391832PYU
CSB-BP391832PYU
CSB-MP391832PYU
CSB-EP391832PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP391833PYU
CSB-EP391833PYU
CSB-BP391833PYU
CSB-MP391833PYU
CSB-EP391833PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP391834PYU
CSB-EP391834PYU
CSB-BP391834PYU
CSB-MP391834PYU
CSB-EP391834PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Ferredoxin--NADP reductase (Pnuc_0382) CSB-YP391835PYU
CSB-EP391835PYU
CSB-BP391835PYU
CSB-MP391835PYU
CSB-EP391835PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Elongation factor 4 (lepA), partial CSB-YP391836PYU
CSB-EP391836PYU
CSB-BP391836PYU
CSB-MP391836PYU
CSB-EP391836PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Elongation factor P (efp) CSB-YP391837PYU
CSB-EP391837PYU
CSB-BP391837PYU
CSB-MP391837PYU
CSB-EP391837PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S6 (rpsF) CSB-YP391838PYU
CSB-EP391838PYU
CSB-BP391838PYU
CSB-MP391838PYU
CSB-EP391838PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S16 (rpsP) CSB-YP391839PYU
CSB-EP391839PYU
CSB-BP391839PYU
CSB-MP391839PYU
CSB-EP391839PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Translation initiation factor IF-1 2 (infA2) CSB-YP391840PYU
CSB-EP391840PYU
CSB-BP391840PYU
CSB-MP391840PYU
CSB-EP391840PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP391841PYU1
CSB-EP391841PYU1
CSB-BP391841PYU1
CSB-MP391841PYU1
CSB-EP391841PYU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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