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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aeromonas salmonicida 30S ribosomal protein S14 (rpsN) CSB-YP391802AUI
CSB-EP391802AUI
CSB-BP391802AUI
CSB-MP391802AUI
CSB-EP391802AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L22 (rplV) CSB-YP391803AUI
CSB-EP391803AUI
CSB-BP391803AUI
CSB-MP391803AUI
CSB-EP391803AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 50S ribosomal protein L4 (rplD) CSB-YP391804AUI
CSB-EP391804AUI
CSB-BP391804AUI
CSB-MP391804AUI
CSB-EP391804AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP391805AUI
CSB-EP391805AUI
CSB-BP391805AUI
CSB-MP391805AUI
CSB-EP391805AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Shikimate dehydrogenase (aroE) CSB-YP391806AUI
CSB-EP391806AUI
CSB-BP391806AUI
CSB-MP391806AUI
CSB-EP391806AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Protein smg homolog (smg) CSB-YP391807AUI
CSB-EP391807AUI
CSB-BP391807AUI
CSB-MP391807AUI
CSB-EP391807AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP391808AUI
CSB-EP391808AUI
CSB-BP391808AUI
CSB-MP391808AUI
CSB-EP391808AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable chorismate--pyruvate lyase (ubiC) CSB-YP391809AUI
CSB-EP391809AUI
CSB-BP391809AUI
CSB-MP391809AUI
CSB-EP391809AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0758 protein ASA_4229 (ASA_4229) CSB-YP391810AUI
CSB-EP391810AUI
CSB-BP391810AUI
CSB-MP391810AUI
CSB-EP391810AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP391811AUI
CSB-EP391811AUI
CSB-BP391811AUI
CSB-MP391811AUI
CSB-EP391811AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP391812AUI
CSB-EP391812AUI
CSB-BP391812AUI
CSB-MP391812AUI
CSB-EP391812AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida ATP-dependent RNA helicase rhlB (rhlB) CSB-YP391813AUI
CSB-EP391813AUI
CSB-BP391813AUI
CSB-MP391813AUI
CSB-EP391813AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP391814AUI1
CSB-EP391814AUI1
CSB-BP391814AUI1
CSB-MP391814AUI1
CSB-EP391814AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida ATP synthase subunit c (atpE), partial CSB-YP391815AUI1
CSB-EP391815AUI1
CSB-BP391815AUI1
CSB-MP391815AUI1
CSB-EP391815AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Membrane protein insertase YidC (yidC), partial CSB-YP391816AUI1
CSB-EP391816AUI1
CSB-BP391816AUI1
CSB-MP391816AUI1
CSB-EP391816AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP391817PYU
CSB-EP391817PYU
CSB-BP391817PYU
CSB-MP391817PYU
CSB-EP391817PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. ATP synthase gamma chain (atpG) CSB-YP391818PYU
CSB-EP391818PYU
CSB-BP391818PYU
CSB-MP391818PYU
CSB-EP391818PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. ATP synthase epsilon chain (atpC) CSB-YP391819PYU
CSB-EP391819PYU
CSB-BP391819PYU
CSB-MP391819PYU
CSB-EP391819PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S12 (rpsL) CSB-YP391820PYU
CSB-EP391820PYU
CSB-BP391820PYU
CSB-MP391820PYU
CSB-EP391820PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L3 (rplC) CSB-YP391821PYU
CSB-EP391821PYU
CSB-BP391821PYU
CSB-MP391821PYU
CSB-EP391821PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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