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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. tularensis 50S ribosomal protein L19 (rplS) CSB-YP393002FDX
CSB-EP393002FDX
CSB-BP393002FDX
CSB-MP393002FDX
CSB-EP393002FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 60 kDa chaperonin (groL), partial CSB-YP393003FDX
CSB-BP393003FDX
CSB-MP393003FDX
CSB-EP393003FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP393004FDX
CSB-EP393004FDX
CSB-BP393004FDX
CSB-MP393004FDX
CSB-EP393004FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis DNA-directed RNA polymerase subunit alpha 2 (rpoA2) CSB-YP393005FDX
CSB-EP393005FDX
CSB-BP393005FDX
CSB-MP393005FDX
CSB-EP393005FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Aspartate 1-decarboxylase (panD) CSB-YP393006FDX
CSB-EP393006FDX
CSB-BP393006FDX
CSB-MP393006FDX
CSB-EP393006FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Pantothenate synthetase (panC) CSB-YP393007FDX
CSB-EP393007FDX
CSB-BP393007FDX
CSB-MP393007FDX
CSB-EP393007FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Phosphoglycerate kinase (pgk) CSB-YP393008FDX
CSB-EP393008FDX
CSB-BP393008FDX
CSB-MP393008FDX
CSB-EP393008FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Chaperone protein DnaK (dnaK), partial CSB-YP393009FDX
CSB-EP393009FDX
CSB-BP393009FDX
CSB-MP393009FDX
CSB-EP393009FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Threonine--tRNA ligase (thrS), partial CSB-YP393010FDX
CSB-EP393010FDX
CSB-BP393010FDX
CSB-MP393010FDX
CSB-EP393010FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 50S ribosomal protein L20 (rplT) CSB-YP393011FDX
CSB-EP393011FDX
CSB-BP393011FDX
CSB-MP393011FDX
CSB-EP393011FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP393012FDX
CSB-EP393012FDX
CSB-BP393012FDX
CSB-MP393012FDX
CSB-EP393012FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis DNA mismatch repair protein MutS (mutS), partial CSB-YP393013FDX
CSB-EP393013FDX
CSB-BP393013FDX
CSB-MP393013FDX
CSB-EP393013FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis UPF0145 protein FTW_0852 (FTW_0852) CSB-YP393014FDX
CSB-EP393014FDX
CSB-BP393014FDX
CSB-MP393014FDX
CSB-EP393014FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Leucine--tRNA ligase (leuS), partial CSB-YP393015FDX
CSB-EP393015FDX
CSB-BP393015FDX
CSB-MP393015FDX
CSB-EP393015FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP393016FDX
CSB-EP393016FDX
CSB-BP393016FDX
CSB-MP393016FDX
CSB-EP393016FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis tRNA 2-thiocytidine biosynthesis protein TtcA 1 (ttcA1) CSB-YP393017FDX
CSB-EP393017FDX
CSB-BP393017FDX
CSB-MP393017FDX
CSB-EP393017FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP393018FDX
CSB-EP393018FDX
CSB-BP393018FDX
CSB-MP393018FDX
CSB-EP393018FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP393019FDX
CSB-EP393019FDX
CSB-BP393019FDX
CSB-MP393019FDX
CSB-EP393019FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP393020FDX
CSB-EP393020FDX
CSB-BP393020FDX
CSB-MP393020FDX
CSB-EP393020FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Putative 3-methyladenine DNA glycosylase (FTW_1062) CSB-YP393021FDX
CSB-EP393021FDX
CSB-BP393021FDX
CSB-MP393021FDX
CSB-EP393021FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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