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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S5P (rps5p) CSB-YP394403PZU
CSB-EP394403PZU
CSB-BP394403PZU
CSB-MP394403PZU
CSB-EP394403PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Nicotinamide-nucleotide adenylyltransferase (Pars_0405) CSB-YP394404PZU
CSB-EP394404PZU
CSB-BP394404PZU
CSB-MP394404PZU
CSB-EP394404PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Histidine--tRNA ligase (hisS) CSB-YP394405PZU
CSB-EP394405PZU
CSB-BP394405PZU
CSB-MP394405PZU
CSB-EP394405PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S19P (rps19p) CSB-YP394406PZU
CSB-EP394406PZU
CSB-BP394406PZU
CSB-MP394406PZU
CSB-EP394406PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Proline--tRNA ligase (proS) CSB-YP394407PZU
CSB-EP394407PZU
CSB-BP394407PZU
CSB-MP394407PZU
CSB-EP394407PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0282 protein Pars_1056 (Pars_1056) CSB-YP394408PZU
CSB-EP394408PZU
CSB-BP394408PZU
CSB-MP394408PZU
CSB-EP394408PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Methionine--tRNA ligase (metG), partial CSB-YP394409PZU
CSB-EP394409PZU
CSB-BP394409PZU
CSB-MP394409PZU
CSB-EP394409PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum GTP cyclohydrolase III (gch3) CSB-YP394410PZU
CSB-EP394410PZU
CSB-BP394410PZU
CSB-MP394410PZU
CSB-EP394410PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP394411PZU
CSB-EP394411PZU
CSB-BP394411PZU
CSB-MP394411PZU
CSB-EP394411PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP394412PZU
CSB-EP394412PZU
CSB-BP394412PZU
CSB-MP394412PZU
CSB-EP394412PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein LX (rplX) CSB-YP394413PZU
CSB-EP394413PZU
CSB-BP394413PZU
CSB-MP394413PZU
CSB-EP394413PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable tRNA pseudouridine synthase D (truD) CSB-YP394414PZU
CSB-EP394414PZU
CSB-BP394414PZU
CSB-MP394414PZU
CSB-EP394414PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S8e (rps8e) CSB-YP394415PZU
CSB-EP394415PZU
CSB-BP394415PZU
CSB-MP394415PZU
CSB-EP394415PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Arginine--tRNA ligase (argS), partial CSB-YP394416PZU
CSB-EP394416PZU
CSB-BP394416PZU
CSB-MP394416PZU
CSB-EP394416PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S4P (rps4p) CSB-YP394417PZU
CSB-EP394417PZU
CSB-BP394417PZU
CSB-MP394417PZU
CSB-EP394417PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP394418PZU
CSB-EP394418PZU
CSB-BP394418PZU
CSB-MP394418PZU
CSB-EP394418PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable porphobilinogen deaminase (hemC) CSB-YP394419PZU
CSB-EP394419PZU
CSB-BP394419PZU
CSB-MP394419PZU
CSB-EP394419PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum DNA-directed RNA polymerase subunit N (rpoN) CSB-YP394420PZU
CSB-EP394420PZU
CSB-BP394420PZU
CSB-MP394420PZU
CSB-EP394420PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP394421RLG
CSB-EP394421RLG
CSB-BP394421RLG
CSB-MP394421RLG
CSB-EP394421RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Dihydrodipicolinate synthase (dapA) CSB-YP394422RLG
CSB-EP394422RLG
CSB-BP394422RLG
CSB-MP394422RLG
CSB-EP394422RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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