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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. tularensis Probable transcriptional regulatory protein FTW_1073 (FTW_1073) CSB-YP393022FDX
CSB-EP393022FDX
CSB-BP393022FDX
CSB-MP393022FDX
CSB-EP393022FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Protein hfq (hfq) CSB-YP393023FDX
CSB-EP393023FDX
CSB-BP393023FDX
CSB-MP393023FDX
CSB-EP393023FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Trigger factor (tig) CSB-YP393024FDX
CSB-EP393024FDX
CSB-BP393024FDX
CSB-MP393024FDX
CSB-EP393024FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 3-dehydroquinate synthase (aroB) CSB-YP393025FDX
CSB-EP393025FDX
CSB-BP393025FDX
CSB-MP393025FDX
CSB-EP393025FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis tRNA 2-thiocytidine biosynthesis protein TtcA 2 (ttcA2) CSB-YP393026FDX
CSB-EP393026FDX
CSB-BP393026FDX
CSB-MP393026FDX
CSB-EP393026FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Isoleucine--tRNA ligase (ileS), partial CSB-YP393027FDX
CSB-EP393027FDX
CSB-BP393027FDX
CSB-MP393027FDX
CSB-EP393027FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis UPF0102 protein FTW_1281 (FTW_1281) CSB-YP393028FDX
CSB-EP393028FDX
CSB-BP393028FDX
CSB-MP393028FDX
CSB-EP393028FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribonuclease PH (rph) CSB-YP393029FDX
CSB-EP393029FDX
CSB-BP393029FDX
CSB-MP393029FDX
CSB-EP393029FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis UPF0176 protein FTW_1391 (FTW_1391) CSB-YP393030FDX
CSB-EP393030FDX
CSB-BP393030FDX
CSB-MP393030FDX
CSB-EP393030FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Alanine racemase (alr) CSB-YP393031FDX
CSB-EP393031FDX
CSB-BP393031FDX
CSB-MP393031FDX
CSB-EP393031FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Protein translocase subunit SecA (secA), partial CSB-YP393032FDX
CSB-EP393032FDX
CSB-BP393032FDX
CSB-MP393032FDX
CSB-EP393032FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Catalase-peroxidase (katG), partial CSB-YP393033FDX
CSB-EP393033FDX
CSB-BP393033FDX
CSB-MP393033FDX
CSB-EP393033FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP393034FDX
CSB-EP393034FDX
CSB-BP393034FDX
CSB-MP393034FDX
CSB-EP393034FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP393035FDX
CSB-EP393035FDX
CSB-BP393035FDX
CSB-MP393035FDX
CSB-EP393035FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP393036FDX
CSB-EP393036FDX
CSB-BP393036FDX
CSB-MP393036FDX
CSB-EP393036FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP393037FDX
CSB-EP393037FDX
CSB-BP393037FDX
CSB-MP393037FDX
CSB-EP393037FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Bifunctional protein GlmU (glmU) CSB-YP393038FDX
CSB-EP393038FDX
CSB-BP393038FDX
CSB-MP393038FDX
CSB-EP393038FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Chaperone protein htpG (htpG), partial CSB-YP393039FDX
CSB-EP393039FDX
CSB-BP393039FDX
CSB-MP393039FDX
CSB-EP393039FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 30S ribosomal protein S11 (rpsK) CSB-YP393040FDX
CSB-EP393040FDX
CSB-BP393040FDX
CSB-MP393040FDX
CSB-EP393040FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 30S ribosomal protein S13 (rpsM) CSB-YP393041FDX
CSB-EP393041FDX
CSB-BP393041FDX
CSB-MP393041FDX
CSB-EP393041FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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