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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas stutzeri Phosphomethylpyrimidine synthase (thiC), partial CSB-YP394604PZP
CSB-EP394604PZP
CSB-BP394604PZP
CSB-MP394604PZP
CSB-EP394604PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri DNA ligase B (ligB), partial CSB-YP394605PZP
CSB-EP394605PZP
CSB-BP394605PZP
CSB-MP394605PZP
CSB-EP394605PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP394606PZP
CSB-EP394606PZP
CSB-BP394606PZP
CSB-MP394606PZP
CSB-EP394606PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP394607PZP
CSB-EP394607PZP
CSB-BP394607PZP
CSB-MP394607PZP
CSB-EP394607PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Probable 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP394608PZP
CSB-EP394608PZP
CSB-BP394608PZP
CSB-MP394608PZP
CSB-EP394608PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP394609PZP
CSB-EP394609PZP
CSB-BP394609PZP
CSB-MP394609PZP
CSB-EP394609PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Peptidyl-tRNA hydrolase (pth) CSB-YP394610SUS
CSB-EP394610SUS
CSB-BP394610SUS
CSB-MP394610SUS
CSB-EP394610SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0297 protein SSU05_0066 (SSU05_0066) CSB-YP394611SUS
CSB-EP394611SUS
CSB-BP394611SUS
CSB-MP394611SUS
CSB-EP394611SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L4 (rplD) CSB-YP394612SUS
CSB-EP394612SUS
CSB-BP394612SUS
CSB-MP394612SUS
CSB-EP394612SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L16 (rplP) CSB-YP394613SUS
CSB-EP394613SUS
CSB-BP394613SUS
CSB-MP394613SUS
CSB-EP394613SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L18 (rplR) CSB-YP394614SUS
CSB-EP394614SUS
CSB-BP394614SUS
CSB-MP394614SUS
CSB-EP394614SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP394615SUS
CSB-EP394615SUS
CSB-BP394615SUS
CSB-MP394615SUS
CSB-EP394615SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0210 protein SSU05_0296 (SSU05_0296) CSB-YP394616SUS
CSB-EP394616SUS
CSB-BP394616SUS
CSB-MP394616SUS
CSB-EP394616SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP394617SUS
CSB-EP394617SUS
CSB-BP394617SUS
CSB-MP394617SUS
CSB-EP394617SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis S-ribosylhomocysteine lyase (luxS) CSB-YP394618SUS
CSB-EP394618SUS
CSB-BP394618SUS
CSB-MP394618SUS
CSB-EP394618SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP394619SUS
CSB-EP394619SUS
CSB-BP394619SUS
CSB-MP394619SUS
CSB-EP394619SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Glutamate 5-kinase (proB) CSB-YP394620SUS
CSB-EP394620SUS
CSB-BP394620SUS
CSB-MP394620SUS
CSB-EP394620SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Dihydrodipicolinate synthase (dapA) CSB-YP394621SUS
CSB-EP394621SUS
CSB-BP394621SUS
CSB-MP394621SUS
CSB-EP394621SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Aspartate carbamoyltransferase (pyrB) CSB-YP394622SUS
CSB-EP394622SUS
CSB-BP394622SUS
CSB-MP394622SUS
CSB-EP394622SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0122 protein SSU05_0875 (SSU05_0875) CSB-YP394623SUS
CSB-EP394623SUS
CSB-BP394623SUS
CSB-MP394623SUS
CSB-EP394623SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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