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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum UPF0059 membrane protein cgR_1530 (cgR_1530), partial CSB-YP393242CXG1
CSB-EP393242CXG1
CSB-BP393242CXG1
CSB-MP393242CXG1
CSB-EP393242CXG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP393243CXG
CSB-EP393243CXG
CSB-BP393243CXG
CSB-MP393243CXG
CSB-EP393243CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphoglycerate kinase (pgk) CSB-YP393244CXG
CSB-EP393244CXG
CSB-BP393244CXG
CSB-MP393244CXG
CSB-EP393244CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0042 nucleotide-binding protein cgR_1639 (cgR_1639) CSB-YP393245CXG
CSB-EP393245CXG
CSB-BP393245CXG
CSB-MP393245CXG
CSB-EP393245CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Aspartate carbamoyltransferase (pyrB) CSB-YP393246CXG
CSB-EP393246CXG
CSB-BP393246CXG
CSB-MP393246CXG
CSB-EP393246CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 3-dehydroquinate synthase (aroB) CSB-YP393247CXG
CSB-EP393247CXG
CSB-BP393247CXG
CSB-MP393247CXG
CSB-EP393247CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP393248CXG
CSB-EP393248CXG
CSB-BP393248CXG
CSB-MP393248CXG
CSB-EP393248CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable transcriptional regulatory protein cgR_1708 (cgR_1708) CSB-YP393249CXG
CSB-EP393249CXG
CSB-BP393249CXG
CSB-MP393249CXG
CSB-EP393249CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP393250CXG
CSB-EP393250CXG
CSB-BP393250CXG
CSB-MP393250CXG
CSB-EP393250CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum LexA repressor (lexA) CSB-YP393251CXG
CSB-EP393251CXG
CSB-BP393251CXG
CSB-MP393251CXG
CSB-EP393251CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP393252CXG
CSB-EP393252CXG
CSB-BP393252CXG
CSB-MP393252CXG
CSB-EP393252CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP393253CXG
CSB-EP393253CXG
CSB-BP393253CXG
CSB-MP393253CXG
CSB-EP393253CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribosome maturation factor RimP (rimP) CSB-YP393254CXG
CSB-EP393254CXG
CSB-BP393254CXG
CSB-MP393254CXG
CSB-EP393254CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L19 (rplS) CSB-YP393255CXG
CSB-EP393255CXG
CSB-BP393255CXG
CSB-MP393255CXG
CSB-EP393255CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Acylphosphatase (acyP) CSB-YP393256CXG
CSB-EP393256CXG
CSB-BP393256CXG
CSB-MP393256CXG
CSB-EP393256CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum DNA polymerase IV (dinB) CSB-YP393257CXG
CSB-EP393257CXG
CSB-BP393257CXG
CSB-MP393257CXG
CSB-EP393257CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP393258CXG
CSB-EP393258CXG
CSB-BP393258CXG
CSB-MP393258CXG
CSB-EP393258CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP393259CXG
CSB-EP393259CXG
CSB-BP393259CXG
CSB-MP393259CXG
CSB-EP393259CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S20 (rpsT) CSB-YP393260CXG
CSB-EP393260CXG
CSB-BP393260CXG
CSB-MP393260CXG
CSB-EP393260CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0272 protein cgR_2381 (cgR_2381) CSB-YP393261CXG
CSB-EP393261CXG
CSB-BP393261CXG
CSB-MP393261CXG
CSB-EP393261CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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