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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. Cell division protein ZapB (zapB) CSB-YP395044EIZ
CSB-EP395044EIZ
CSB-BP395044EIZ
CSB-MP395044EIZ
CSB-EP395044EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein FdhE homolog (fdhE) CSB-YP395045EIZ
CSB-EP395045EIZ
CSB-BP395045EIZ
CSB-MP395045EIZ
CSB-EP395045EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. ATP synthase gamma chain (atpG) CSB-YP395046EIZ
CSB-EP395046EIZ
CSB-BP395046EIZ
CSB-MP395046EIZ
CSB-EP395046EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Transcription factor E (tfe) CSB-YP395047PZU
CSB-EP395047PZU
CSB-BP395047PZU
CSB-MP395047PZU
CSB-EP395047PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Proteasome subunit beta 1 (psmB1) CSB-YP395048PZU
CSB-EP395048PZU
CSB-BP395048PZU
CSB-MP395048PZU
CSB-EP395048PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0215 protein Pars_0148 (Pars_0148) CSB-YP395049PZU
CSB-EP395049PZU
CSB-BP395049PZU
CSB-MP395049PZU
CSB-EP395049PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Ribonuclease HII (rnhB) CSB-YP395050PZU
CSB-EP395050PZU
CSB-BP395050PZU
CSB-MP395050PZU
CSB-EP395050PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Chromatin protein Cren7 (creN7) CSB-YP395051PZU
CSB-EP395051PZU
CSB-BP395051PZU
CSB-MP395051PZU
CSB-EP395051PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Riboflavin kinase (ribK) CSB-YP395052PZU
CSB-EP395052PZU
CSB-BP395052PZU
CSB-MP395052PZU
CSB-EP395052PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0210 protein Pars_1033 (Pars_1033) CSB-YP395053PZU
CSB-EP395053PZU
CSB-BP395053PZU
CSB-MP395053PZU
CSB-EP395053PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Elongation factor 1-alpha (tuf) CSB-YP395054PZU
CSB-EP395054PZU
CSB-BP395054PZU
CSB-MP395054PZU
CSB-EP395054PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Tryptophan--tRNA ligase (trpS) CSB-YP395055PZU
CSB-EP395055PZU
CSB-BP395055PZU
CSB-MP395055PZU
CSB-EP395055PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP395056PZU
CSB-EP395056PZU
CSB-BP395056PZU
CSB-MP395056PZU
CSB-EP395056PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Translation initiation factor 5A (eIF5A) CSB-YP395057PZU
CSB-EP395057PZU
CSB-BP395057PZU
CSB-MP395057PZU
CSB-EP395057PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S27e (rps27e) CSB-YP395058PZU
CSB-EP395058PZU
CSB-BP395058PZU
CSB-MP395058PZU
CSB-EP395058PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L15P (rpl15p) CSB-YP395059PZU
CSB-EP395059PZU
CSB-BP395059PZU
CSB-MP395059PZU
CSB-EP395059PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0095 protein Pars_1886 (Pars_1886) CSB-YP395060PZU
CSB-EP395060PZU
CSB-BP395060PZU
CSB-MP395060PZU
CSB-EP395060PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0201 protein Pars_1985 (Pars_1985) CSB-YP395061PZU
CSB-EP395061PZU
CSB-BP395061PZU
CSB-MP395061PZU
CSB-EP395061PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L3P (rpl3p) CSB-YP395062PZU
CSB-EP395062PZU
CSB-BP395062PZU
CSB-MP395062PZU
CSB-EP395062PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP395063PZU
CSB-EP395063PZU
CSB-BP395063PZU
CSB-MP395063PZU
CSB-EP395063PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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