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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lobularia maritima Putative membrane protein ycf1 (ycf1-A), partial CSB-YP393442LLU
CSB-EP393442LLU
CSB-BP393442LLU
CSB-MP393442LLU
CSB-EP393442LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Maturase K (matK), partial CSB-YP393443NEV
CSB-EP393443NEV
CSB-BP393443NEV
CSB-MP393443NEV
CSB-EP393443NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP393444NEV
CSB-EP393444NEV
CSB-BP393444NEV
CSB-MP393444NEV
CSB-EP393444NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Photosystem II reaction center protein M (psbM), partial CSB-YP393445NEV
CSB-EP393445NEV
CSB-BP393445NEV
CSB-MP393445NEV
CSB-EP393445NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP393446NEV1
CSB-EP393446NEV1
CSB-BP393446NEV1
CSB-MP393446NEV1
CSB-EP393446NEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP393447NEV
CSB-EP393447NEV
CSB-BP393447NEV
CSB-MP393447NEV
CSB-EP393447NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD) CSB-YP393448NEV
CSB-EP393448NEV
CSB-BP393448NEV
CSB-MP393448NEV
CSB-EP393448NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Apocytochrome f (petA), partial CSB-YP393449NEV1
CSB-EP393449NEV1
CSB-BP393449NEV1
CSB-MP393449NEV1
CSB-EP393449NEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Photosystem I reaction center subunit IX (psaJ), partial CSB-YP393450NEV
CSB-EP393450NEV
CSB-BP393450NEV
CSB-MP393450NEV
CSB-EP393450NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP393451NEV
CSB-EP393451NEV
CSB-BP393451NEV
CSB-MP393451NEV
CSB-EP393451NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Protein psbN (psbN), partial CSB-YP393452NEV
CSB-EP393452NEV
CSB-BP393452NEV
CSB-MP393452NEV
CSB-EP393452NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP393453NEV
CSB-EP393453NEV
CSB-BP393453NEV
CSB-MP393453NEV
CSB-EP393453NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP393454NEV
CSB-EP393454NEV
CSB-BP393454NEV
CSB-MP393454NEV
CSB-EP393454NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP393455NEV
CSB-EP393455NEV
CSB-BP393455NEV
CSB-MP393455NEV
CSB-EP393455NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Photosystem I iron-sulfur center (psaC) CSB-YP393456NEV
CSB-EP393456NEV
CSB-BP393456NEV
CSB-MP393456NEV
CSB-EP393456NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP393457NEV
CSB-EP393457NEV
CSB-BP393457NEV
CSB-MP393457NEV
CSB-EP393457NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus koraiensis Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP393458PYR
CSB-EP393458PYR
CSB-BP393458PYR
CSB-MP393458PYR
CSB-EP393458PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WD repeat-containing protein mio-B, partial CSB-YP393459XBE
CSB-EP393459XBE
CSB-BP393459XBE
CSB-MP393459XBE
CSB-EP393459XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio CTD small phosphatase-like protein 2-B (ctdspl2b) CSB-YP393460DIL
CSB-EP393460DIL
CSB-BP393460DIL
CSB-MP393460DIL
CSB-EP393460DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio tRNA (uracil (54)-C (5))-methyltransferase homolog-B CSB-YP393461DIL
CSB-EP393461DIL
CSB-BP393461DIL
CSB-MP393461DIL
CSB-EP393461DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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