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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis Octanoyltransferase (lipB) CSB-YP393842YAL
CSB-EP393842YAL
CSB-BP393842YAL
CSB-MP393842YAL
CSB-EP393842YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Chaperone protein htpG (htpG), partial CSB-YP393843YAL
CSB-EP393843YAL
CSB-BP393843YAL
CSB-MP393843YAL
CSB-EP393843YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA sulfurtransferase (thiI) CSB-YP393844YAL
CSB-EP393844YAL
CSB-BP393844YAL
CSB-MP393844YAL
CSB-EP393844YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Shikimate kinase 2 (aroL) CSB-YP393845YAL
CSB-EP393845YAL
CSB-BP393845YAL
CSB-MP393845YAL
CSB-EP393845YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DNA polymerase IV (dinB) CSB-YP393846YAL
CSB-EP393846YAL
CSB-BP393846YAL
CSB-MP393846YAL
CSB-EP393846YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acireductone dioxygenase (mtnD) CSB-YP393847YAL
CSB-EP393847YAL
CSB-BP393847YAL
CSB-MP393847YAL
CSB-EP393847YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Aspartate 1-decarboxylase (panD) CSB-YP393848YAL
CSB-EP393848YAL
CSB-BP393848YAL
CSB-MP393848YAL
CSB-EP393848YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP393849YAL
CSB-EP393849YAL
CSB-BP393849YAL
CSB-MP393849YAL
CSB-EP393849YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein RecA (recA) CSB-YP393850YAL
CSB-EP393850YAL
CSB-BP393850YAL
CSB-MP393850YAL
CSB-EP393850YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S16 (rpsP) CSB-YP393851YAL
CSB-EP393851YAL
CSB-BP393851YAL
CSB-MP393851YAL
CSB-EP393851YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein translocase subunit SecA (secA), partial CSB-YP393852YAL
CSB-EP393852YAL
CSB-BP393852YAL
CSB-MP393852YAL
CSB-EP393852YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP393853YAL
CSB-EP393853YAL
CSB-BP393853YAL
CSB-MP393853YAL
CSB-EP393853YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Chaperone protein DnaK (dnaK), partial CSB-YP393854YAL
CSB-EP393854YAL
CSB-BP393854YAL
CSB-MP393854YAL
CSB-EP393854YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0391 membrane protein YPDSF_3201 (YPDSF_3201), partial CSB-YP393855YAL
CSB-EP393855YAL
CSB-BP393855YAL
CSB-MP393855YAL
CSB-EP393855YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Uncharacterized aldolase lsrF (lsrF) CSB-YP393856YAL
CSB-EP393856YAL
CSB-BP393856YAL
CSB-MP393856YAL
CSB-EP393856YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP393857YAL
CSB-EP393857YAL
CSB-BP393857YAL
CSB-MP393857YAL
CSB-EP393857YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP393858YAL
CSB-EP393858YAL
CSB-BP393858YAL
CSB-MP393858YAL
CSB-EP393858YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein CyaY (cyaY) CSB-YP393859YAL
CSB-EP393859YAL
CSB-BP393859YAL
CSB-MP393859YAL
CSB-EP393859YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis HTH-type transcriptional regulator hdfR (hdfR) CSB-YP393860YAL
CSB-EP393860YAL
CSB-BP393860YAL
CSB-MP393860YAL
CSB-EP393860YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP393861YAL
CSB-EP393861YAL
CSB-BP393861YAL
CSB-MP393861YAL
CSB-EP393861YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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