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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Metallosphaera sedula Probable molybdenum cofactor biosynthesis protein A (moaA) CSB-YP396805MNM
CSB-EP396805MNM
CSB-BP396805MNM
CSB-MP396805MNM
CSB-EP396805MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Arginine decarboxylase proenzyme (Msed_1645) CSB-YP396806MNM
CSB-EP396806MNM
CSB-BP396806MNM
CSB-MP396806MNM
CSB-EP396806MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP396807MNM
CSB-EP396807MNM
CSB-BP396807MNM
CSB-MP396807MNM
CSB-EP396807MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Translation initiation factor 5A (eIF5A) CSB-YP396808MNM
CSB-EP396808MNM
CSB-BP396808MNM
CSB-MP396808MNM
CSB-EP396808MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Uridylate kinase (pyrH) CSB-YP396809MNM
CSB-EP396809MNM
CSB-BP396809MNM
CSB-MP396809MNM
CSB-EP396809MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula V-type ATP synthase alpha chain (atpA), partial CSB-YP396810MNM
CSB-EP396810MNM
CSB-BP396810MNM
CSB-MP396810MNM
CSB-EP396810MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP396811MNM
CSB-EP396811MNM
CSB-BP396811MNM
CSB-MP396811MNM
CSB-EP396811MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Carbamoyl-phosphate synthase small chain (carA) CSB-YP396812MNM
CSB-EP396812MNM
CSB-BP396812MNM
CSB-MP396812MNM
CSB-EP396812MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Probable thymidylate kinase (tmk) CSB-YP396813MNM
CSB-EP396813MNM
CSB-BP396813MNM
CSB-MP396813MNM
CSB-EP396813MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Proteasome subunit beta 1 (psmB1) CSB-YP396814MNM
CSB-EP396814MNM
CSB-BP396814MNM
CSB-MP396814MNM
CSB-EP396814MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP396815MNM
CSB-EP396815MNM
CSB-BP396815MNM
CSB-MP396815MNM
CSB-EP396815MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula UPF0095 protein Msed_2153 (Msed_2153) CSB-YP396816MNM
CSB-EP396816MNM
CSB-BP396816MNM
CSB-MP396816MNM
CSB-EP396816MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP396817MNM
CSB-EP396817MNM
CSB-BP396817MNM
CSB-MP396817MNM
CSB-EP396817MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP396818BPF
CSB-EP396818BPF
CSB-BP396818BPF
CSB-MP396818BPF
CSB-EP396818BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S15 (rpsO) CSB-YP396819BPF
CSB-EP396819BPF
CSB-BP396819BPF
CSB-MP396819BPF
CSB-EP396819BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP396820BPF
CSB-EP396820BPF
CSB-BP396820BPF
CSB-MP396820BPF
CSB-EP396820BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L35 (rpmI) CSB-YP396821BPF
CSB-EP396821BPF
CSB-BP396821BPF
CSB-MP396821BPF
CSB-EP396821BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Leucine--tRNA ligase (leuS), partial CSB-YP396822BPF
CSB-EP396822BPF
CSB-BP396822BPF
CSB-MP396822BPF
CSB-EP396822BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP-dependent protease subunit HslV (hslV) CSB-YP396823BPF
CSB-EP396823BPF
CSB-BP396823BPF
CSB-MP396823BPF
CSB-EP396823BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Bifunctional protein FolD (folD) CSB-YP396824BPF
CSB-EP396824BPF
CSB-BP396824BPF
CSB-MP396824BPF
CSB-EP396824BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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