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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens 30S ribosomal protein S18 (rpsR) CSB-YP396765STQ
CSB-EP396765STQ
CSB-BP396765STQ
CSB-MP396765STQ
CSB-EP396765STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens S-adenosylmethionine synthase (metK) CSB-YP396766STQ
CSB-EP396766STQ
CSB-BP396766STQ
CSB-MP396766STQ
CSB-EP396766STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein ApaG (apaG) CSB-YP396767STQ
CSB-EP396767STQ
CSB-BP396767STQ
CSB-MP396767STQ
CSB-EP396767STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP396768STQ1
CSB-EP396768STQ1
CSB-BP396768STQ1
CSB-MP396768STQ1
CSB-EP396768STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Na (+)/H (+) antiporter nhaA, partial CSB-YP396769STQ
CSB-EP396769STQ
CSB-BP396769STQ
CSB-MP396769STQ
CSB-EP396769STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosome maturation factor RimM (rimM) CSB-YP396770STQ
CSB-EP396770STQ
CSB-BP396770STQ
CSB-MP396770STQ
CSB-EP396770STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP396771STQ
CSB-EP396771STQ
CSB-BP396771STQ
CSB-MP396771STQ
CSB-EP396771STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Recombination-associated protein rdgC (rdgC) CSB-YP396772STQ
CSB-EP396772STQ
CSB-BP396772STQ
CSB-MP396772STQ
CSB-EP396772STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens GTP cyclohydrolase-2 (ribA) CSB-YP396773STQ
CSB-EP396773STQ
CSB-BP396773STQ
CSB-MP396773STQ
CSB-EP396773STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens D-alanine--D-alanine ligase (ddl) CSB-YP396774STQ
CSB-EP396774STQ
CSB-BP396774STQ
CSB-MP396774STQ
CSB-EP396774STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Electron transport complex protein RnfE (rnfE), partial CSB-YP396775STQ1
CSB-EP396775STQ1
CSB-BP396775STQ1
CSB-MP396775STQ1
CSB-EP396775STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Elongation factor P (efp) CSB-YP396776STQ
CSB-EP396776STQ
CSB-BP396776STQ
CSB-MP396776STQ
CSB-EP396776STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP396777STQ
CSB-EP396777STQ
CSB-BP396777STQ
CSB-MP396777STQ
CSB-EP396777STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Cytidylate kinase (cmk) CSB-YP396778STQ
CSB-EP396778STQ
CSB-BP396778STQ
CSB-MP396778STQ
CSB-EP396778STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Chaperone protein hscA homolog (hscA), partial CSB-YP396779STQ
CSB-EP396779STQ
CSB-BP396779STQ
CSB-MP396779STQ
CSB-EP396779STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP396780STQ
CSB-EP396780STQ
CSB-BP396780STQ
CSB-MP396780STQ
CSB-EP396780STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Anthranilate phosphoribosyltransferase (trpD) CSB-YP396781STQ
CSB-EP396781STQ
CSB-BP396781STQ
CSB-MP396781STQ
CSB-EP396781STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP396782STQ
CSB-EP396782STQ
CSB-BP396782STQ
CSB-MP396782STQ
CSB-EP396782STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glutamate--tRNA ligase (gltX) CSB-YP396783STQ
CSB-EP396783STQ
CSB-BP396783STQ
CSB-MP396783STQ
CSB-EP396783STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Histidine--tRNA ligase (hisS) CSB-YP396784STQ
CSB-EP396784STQ
CSB-BP396784STQ
CSB-MP396784STQ
CSB-EP396784STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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