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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF56 (ORF56) CSB-YP396865ATY
CSB-EP396865ATY
CSB-BP396865ATY
CSB-MP396865ATY
CSB-EP396865ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF99a (ORF99a) CSB-YP396866ATY
CSB-EP396866ATY
CSB-BP396866ATY
CSB-MP396866ATY
CSB-EP396866ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF257 (ORF257) CSB-YP396867ATY
CSB-EP396867ATY
CSB-BP396867ATY
CSB-MP396867ATY
CSB-EP396867ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF167 (ORF167) CSB-YP396868ATY
CSB-EP396868ATY
CSB-BP396868ATY
CSB-MP396868ATY
CSB-EP396868ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF150a (ORF150a) CSB-YP396869ATY
CSB-EP396869ATY
CSB-BP396869ATY
CSB-MP396869ATY
CSB-EP396869ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Avenin-like b4 CSB-YP396870TQN
CSB-EP396870TQN
CSB-BP396870TQN
CSB-MP396870TQN
CSB-EP396870TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ymgI (ymgI) CSB-YP396871ENV
CSB-EP396871ENV
CSB-BP396871ENV
CSB-MP396871ENV
CSB-EP396871ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yncL (yncL), partial CSB-YP396872ENV
CSB-EP396872ENV
CSB-BP396872ENV
CSB-MP396872ENV
CSB-EP396872ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Keratin, type II cytoskeletal 1 (KRT1), partial CSB-YP396873EQV
CSB-EP396873EQV
CSB-BP396873EQV
CSB-MP396873EQV
CSB-EP396873EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Keratin, type I cytoskeletal 25 (KRT25) CSB-YP396874EQV
CSB-EP396874EQV
CSB-BP396874EQV
CSB-MP396874EQV
CSB-EP396874EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase dbp8 (dbp8), partial CSB-YP396875AVE
CSB-EP396875AVE
CSB-BP396875AVE
CSB-MP396875AVE
CSB-EP396875AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger U3 small nucleolar RNA-associated protein 10 (utp10), partial CSB-YP396876AVE
CSB-EP396876AVE
CSB-BP396876AVE
CSB-MP396876AVE
CSB-EP396876AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Cyanate hydratase (CYN) CSB-YP396877VFQ
CSB-EP396877VFQ
CSB-BP396877VFQ
CSB-MP396877VFQ
CSB-EP396877VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Aspartate--tRNA ligase (aspS), partial CSB-YP396878ODJ
CSB-EP396878ODJ
CSB-BP396878ODJ
CSB-MP396878ODJ
CSB-EP396878ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Probable transcriptional regulatory protein OTBS_0251 (OTBS_0251) CSB-YP396879ODJ
CSB-EP396879ODJ
CSB-BP396879ODJ
CSB-MP396879ODJ
CSB-EP396879ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S5 (rpsE) CSB-YP396880ODJ
CSB-EP396880ODJ
CSB-BP396880ODJ
CSB-MP396880ODJ
CSB-EP396880ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L14 (rplN) CSB-YP396881ODJ
CSB-EP396881ODJ
CSB-BP396881ODJ
CSB-MP396881ODJ
CSB-EP396881ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S19 (rpsS) CSB-YP396882ODJ
CSB-EP396882ODJ
CSB-BP396882ODJ
CSB-MP396882ODJ
CSB-EP396882ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Putative Holliday junction resolvase (OTBS_0498) CSB-YP396883ODJ
CSB-EP396883ODJ
CSB-BP396883ODJ
CSB-MP396883ODJ
CSB-EP396883ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Glutamate--tRNA ligase 1 (gltX1) CSB-YP396884ODJ
CSB-EP396884ODJ
CSB-BP396884ODJ
CSB-MP396884ODJ
CSB-EP396884ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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