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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP396845BPF
CSB-EP396845BPF
CSB-BP396845BPF
CSB-MP396845BPF
CSB-EP396845BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP396846BPF
CSB-EP396846BPF
CSB-BP396846BPF
CSB-MP396846BPF
CSB-EP396846BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Arginine--tRNA ligase (argS), partial CSB-YP396847BPF
CSB-EP396847BPF
CSB-BP396847BPF
CSB-MP396847BPF
CSB-EP396847BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Indole-3-glycerol phosphate synthase (trpC) CSB-YP396848BPF
CSB-EP396848BPF
CSB-BP396848BPF
CSB-MP396848BPF
CSB-EP396848BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Heme A synthase (ctaA), partial CSB-YP396849BPF
CSB-EP396849BPF
CSB-BP396849BPF
CSB-MP396849BPF
CSB-EP396849BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. N utilization substance protein B homolog (nusB) CSB-YP396850BPF
CSB-EP396850BPF
CSB-BP396850BPF
CSB-MP396850BPF
CSB-EP396850BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Large-conductance mechanosensitive channel (mscL), partial CSB-YP396851BPF1
CSB-EP396851BPF1
CSB-BP396851BPF1
CSB-MP396851BPF1
CSB-EP396851BPF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable potassium transport system protein kup 2 (kup2), partial CSB-YP396852BPF
CSB-EP396852BPF
CSB-BP396852BPF
CSB-MP396852BPF
CSB-EP396852BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphoheptose isomerase (gmhA) CSB-YP396853BPF
CSB-EP396853BPF
CSB-BP396853BPF
CSB-MP396853BPF
CSB-EP396853BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. DNA ligase (ligA), partial CSB-YP396854BPF
CSB-EP396854BPF
CSB-BP396854BPF
CSB-MP396854BPF
CSB-EP396854BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP396855BPF
CSB-EP396855BPF
CSB-BP396855BPF
CSB-MP396855BPF
CSB-EP396855BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Pyrroloquinoline-quinone synthase (pqqC) CSB-YP396856BPF
CSB-EP396856BPF
CSB-BP396856BPF
CSB-MP396856BPF
CSB-EP396856BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreD 3 (ureD3) CSB-YP396857BPF
CSB-EP396857BPF
CSB-BP396857BPF
CSB-MP396857BPF
CSB-EP396857BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Adenylosuccinate synthetase (purA) CSB-YP396858BPF
CSB-EP396858BPF
CSB-BP396858BPF
CSB-MP396858BPF
CSB-EP396858BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L36 (rpmJ) CSB-YP396859BPF
CSB-EP396859BPF
CSB-BP396859BPF
CSB-MP396859BPF
CSB-EP396859BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Homoserine kinase (thrB) CSB-YP396860BPF
CSB-EP396860BPF
CSB-BP396860BPF
CSB-MP396860BPF
CSB-EP396860BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Protein ApaG (apaG) CSB-YP396861BPF
CSB-EP396861BPF
CSB-BP396861BPF
CSB-MP396861BPF
CSB-EP396861BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 2 CSB-YP396862ROC
CSB-EP396862ROC
CSB-BP396862ROC
CSB-MP396862ROC
CSB-EP396862ROC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP396863ROC
CSB-EP396863ROC
CSB-BP396863ROC
CSB-MP396863ROC
CSB-EP396863ROC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus bottle-shaped virus Uncharacterized protein ORF243 (ORF243) CSB-YP396864ATY
CSB-EP396864ATY
CSB-BP396864ATY
CSB-MP396864ATY
CSB-EP396864ATY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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