Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelotomaculum thermopropionicum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP398826PYG
CSB-EP398826PYG
CSB-BP398826PYG
CSB-MP398826PYG
CSB-EP398826PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP398827PYG
CSB-EP398827PYG
CSB-BP398827PYG
CSB-MP398827PYG
CSB-EP398827PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 3-dehydroquinate synthase (aroB) CSB-YP398828PYG
CSB-EP398828PYG
CSB-BP398828PYG
CSB-MP398828PYG
CSB-EP398828PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP398829PYG
CSB-EP398829PYG
CSB-BP398829PYG
CSB-MP398829PYG
CSB-EP398829PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum ATP phosphoribosyltransferase (hisG) CSB-YP398830PYG
CSB-EP398830PYG
CSB-BP398830PYG
CSB-MP398830PYG
CSB-EP398830PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Cell division topological specificity factor (minE) CSB-YP398831PYG
CSB-EP398831PYG
CSB-BP398831PYG
CSB-MP398831PYG
CSB-EP398831PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Phosphomethylpyrimidine synthase (thiC) CSB-YP398832PYG
CSB-EP398832PYG
CSB-BP398832PYG
CSB-MP398832PYG
CSB-EP398832PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Acetylglutamate kinase (argB) CSB-YP398833PYG
CSB-EP398833PYG
CSB-BP398833PYG
CSB-MP398833PYG
CSB-EP398833PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L36 (rpmJ) CSB-YP398834PYG
CSB-EP398834PYG
CSB-BP398834PYG
CSB-MP398834PYG
CSB-EP398834PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L13 (rplM) CSB-YP398835PYG
CSB-EP398835PYG
CSB-BP398835PYG
CSB-MP398835PYG
CSB-EP398835PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP398836PYG
CSB-EP398836PYG
CSB-BP398836PYG
CSB-MP398836PYG
CSB-EP398836PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Putative ATP:guanido phosphotransferase PTH_0282 (PTH_0282) CSB-YP398837PYG
CSB-EP398837PYG
CSB-BP398837PYG
CSB-MP398837PYG
CSB-EP398837PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Recombination protein RecR (recR) CSB-YP398838PYG
CSB-EP398838PYG
CSB-BP398838PYG
CSB-MP398838PYG
CSB-EP398838PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Iron/zinc purple acid phosphatase-like protein (papl) CSB-YP398839DIL
CSB-EP398839DIL
CSB-BP398839DIL
CSB-MP398839DIL
CSB-EP398839DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Centrosomal protein kizuna (plk1s1), partial CSB-YP398840DIL
CSB-EP398840DIL
CSB-BP398840DIL
CSB-MP398840DIL
CSB-EP398840DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Solute carrier family 25 member 38 homolog (PGUG_00074), partial CSB-YP398841MTM1
CSB-EP398841MTM1
CSB-BP398841MTM1
CSB-MP398841MTM1
CSB-EP398841MTM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Riboflavin kinase (FMN1) CSB-YP398842MTM
CSB-EP398842MTM
CSB-BP398842MTM
CSB-MP398842MTM
CSB-EP398842MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Riboflavin synthase alpha chain (RIB5) CSB-YP398843MTM
CSB-EP398843MTM
CSB-BP398843MTM
CSB-MP398843MTM
CSB-EP398843MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP398844MTM
CSB-EP398844MTM
CSB-BP398844MTM
CSB-MP398844MTM
CSB-EP398844MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase DBP9 (DBP9), partial CSB-YP398845MTM
CSB-EP398845MTM
CSB-BP398845MTM
CSB-MP398845MTM
CSB-EP398845MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>