Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermotoga petrophila Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP400627TKF
CSB-EP400627TKF
CSB-BP400627TKF
CSB-MP400627TKF
CSB-EP400627TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Ribosomal protein L11 methyltransferase (prmA) CSB-YP400628TKF
CSB-EP400628TKF
CSB-BP400628TKF
CSB-MP400628TKF
CSB-EP400628TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP400629TKF
CSB-EP400629TKF
CSB-BP400629TKF
CSB-MP400629TKF
CSB-EP400629TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP400630SUJ
CSB-EP400630SUJ
CSB-BP400630SUJ
CSB-MP400630SUJ
CSB-EP400630SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus FMN-dependent NADH-azoreductase (azoR) CSB-YP400631SUJ
CSB-EP400631SUJ
CSB-BP400631SUJ
CSB-MP400631SUJ
CSB-EP400631SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP400632SUJ
CSB-EP400632SUJ
CSB-BP400632SUJ
CSB-MP400632SUJ
CSB-EP400632SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0753 protein SaurJH9_0475 (SaurJH9_0475), partial CSB-YP400633SUJ
CSB-EP400633SUJ
CSB-BP400633SUJ
CSB-MP400633SUJ
CSB-EP400633SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative septation protein spoVG (spoVG) CSB-YP400634SUJ
CSB-EP400634SUJ
CSB-BP400634SUJ
CSB-MP400634SUJ
CSB-EP400634SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 1 (rpmG1) CSB-YP400635SUJ
CSB-EP400635SUJ
CSB-BP400635SUJ
CSB-MP400635SUJ
CSB-EP400635SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0741 protein SaurJH9_0616 (SaurJH9_0616) CSB-YP400636SUJ
CSB-EP400636SUJ
CSB-BP400636SUJ
CSB-MP400636SUJ
CSB-EP400636SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0178 protein SaurJH9_0705 (SaurJH9_0705) CSB-YP400637SUJ
CSB-EP400637SUJ
CSB-BP400637SUJ
CSB-MP400637SUJ
CSB-EP400637SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein nrdI (nrdI) CSB-YP400638SUJ
CSB-EP400638SUJ
CSB-BP400638SUJ
CSB-MP400638SUJ
CSB-EP400638SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HPr kinase/phosphorylase (hprK) CSB-YP400639SUJ
CSB-EP400639SUJ
CSB-BP400639SUJ
CSB-MP400639SUJ
CSB-EP400639SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 1 CSB-YP400640SUJ
CSB-EP400640SUJ
CSB-BP400640SUJ
CSB-MP400640SUJ
CSB-EP400640SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0738 protein SaurJH9_1003 (SaurJH9_1003) CSB-YP400641SUJ
CSB-EP400641SUJ
CSB-BP400641SUJ
CSB-MP400641SUJ
CSB-EP400641SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP400642SUJ
CSB-EP400642SUJ
CSB-BP400642SUJ
CSB-MP400642SUJ
CSB-EP400642SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0358 protein SaurJH9_1172 (SaurJH9_1172) CSB-YP400643SUJ
CSB-EP400643SUJ
CSB-BP400643SUJ
CSB-MP400643SUJ
CSB-EP400643SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0348 protein SaurJH9_1185 (SaurJH9_1185) CSB-YP400644SUJ
CSB-EP400644SUJ
CSB-BP400644SUJ
CSB-MP400644SUJ
CSB-EP400644SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate racemase (murI) CSB-YP400645SUJ
CSB-EP400645SUJ
CSB-BP400645SUJ
CSB-MP400645SUJ
CSB-EP400645SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional protein pyrR (pyrR) CSB-YP400646SUJ
CSB-EP400646SUJ
CSB-BP400646SUJ
CSB-MP400646SUJ
CSB-EP400646SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>