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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S2 (rpsB) CSB-YP398886PYG
CSB-EP398886PYG
CSB-BP398886PYG
CSB-MP398886PYG
CSB-EP398886PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Ribosome maturation factor RimP (rimP) CSB-YP398887PYG
CSB-EP398887PYG
CSB-BP398887PYG
CSB-MP398887PYG
CSB-EP398887PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP398888PYG
CSB-EP398888PYG
CSB-BP398888PYG
CSB-MP398888PYG
CSB-EP398888PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum UPF0473 protein PTH_1066 (PTH_1066) CSB-YP398889PYG
CSB-EP398889PYG
CSB-BP398889PYG
CSB-MP398889PYG
CSB-EP398889PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Cobyric acid synthase (cobQ), partial CSB-YP398890PYG
CSB-EP398890PYG
CSB-BP398890PYG
CSB-MP398890PYG
CSB-EP398890PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L27 (rpmA) CSB-YP398891PYG
CSB-EP398891PYG
CSB-BP398891PYG
CSB-MP398891PYG
CSB-EP398891PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP398892PYG
CSB-EP398892PYG
CSB-BP398892PYG
CSB-MP398892PYG
CSB-EP398892PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Biotin synthase (bioB) CSB-YP398893PYG
CSB-EP398893PYG
CSB-BP398893PYG
CSB-MP398893PYG
CSB-EP398893PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 4-hydroxy-2-oxovalerate aldolase (PTH_0483) CSB-YP398894PYG
CSB-EP398894PYG
CSB-BP398894PYG
CSB-MP398894PYG
CSB-EP398894PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L17 (rplQ) CSB-YP398895PYG
CSB-EP398895PYG
CSB-BP398895PYG
CSB-MP398895PYG
CSB-EP398895PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L6 (rplF) CSB-YP398896PYG
CSB-EP398896PYG
CSB-BP398896PYG
CSB-MP398896PYG
CSB-EP398896PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L30 (rpmD) CSB-YP398897PYG
CSB-EP398897PYG
CSB-BP398897PYG
CSB-MP398897PYG
CSB-EP398897PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L2 (rplB) CSB-YP398898PYG
CSB-EP398898PYG
CSB-BP398898PYG
CSB-MP398898PYG
CSB-EP398898PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Cysteine--tRNA ligase (cysS) CSB-YP398899PYG
CSB-EP398899PYG
CSB-BP398899PYG
CSB-MP398899PYG
CSB-EP398899PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Bifunctional protein GlmU (glmU) CSB-YP398900PYG
CSB-EP398900PYG
CSB-BP398900PYG
CSB-MP398900PYG
CSB-EP398900PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C1orf170 homolog, partial CSB-YP398901BO
CSB-EP398901BO
CSB-BP398901BO
CSB-MP398901BO
CSB-EP398901BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tyrosyl-DNA phosphodiesterase 2 (tdp2) CSB-YP398902XBE
CSB-EP398902XBE
CSB-BP398902XBE
CSB-MP398902XBE
CSB-EP398902XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATPase synthesis protein 25, mitochondrial (ATP25), partial CSB-YP398903MTM
CSB-EP398903MTM
CSB-BP398903MTM
CSB-MP398903MTM
CSB-EP398903MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP398904MTM
CSB-EP398904MTM
CSB-BP398904MTM
CSB-MP398904MTM
CSB-EP398904MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Increased rDNA silencing protein 4 (IRS4), partial CSB-YP398905MTM
CSB-EP398905MTM
CSB-BP398905MTM
CSB-MP398905MTM
CSB-EP398905MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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