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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Meyerozyma guilliermondii tRNA (guanine-N (7)-)-methyltransferase subunit TRM82 CSB-YP399507MTM
CSB-EP399507MTM
CSB-BP399507MTM
CSB-MP399507MTM
CSB-EP399507MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Long chronological lifespan protein 2 (LCL2) CSB-YP399508MTM
CSB-EP399508MTM
CSB-BP399508MTM
CSB-MP399508MTM
CSB-EP399508MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Fe-S cluster assembly protein DRE2 (DRE2) CSB-YP399509MTM
CSB-EP399509MTM
CSB-BP399509MTM
CSB-MP399509MTM
CSB-EP399509MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Enhancer of translation termination 1 (ETT1) CSB-YP399510MTM
CSB-EP399510MTM
CSB-BP399510MTM
CSB-MP399510MTM
CSB-EP399510MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Glucose starvation modulator protein 1 (GSM1), partial CSB-YP399511MTM
CSB-EP399511MTM
CSB-BP399511MTM
CSB-MP399511MTM
CSB-EP399511MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein SQS1 (SQS1), partial CSB-YP399512LLV
CSB-EP399512LLV
CSB-BP399512LLV
CSB-MP399512LLV
CSB-EP399512LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP399513LLV
CSB-EP399513LLV
CSB-BP399513LLV
CSB-MP399513LLV
CSB-EP399513LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Leukotriene A-4 hydrolase homolog (LELG_00410), partial CSB-YP399514LLV
CSB-EP399514LLV
CSB-BP399514LLV
CSB-MP399514LLV
CSB-EP399514LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Succinate dehydrogenase assembly factor 2, mitochondrial (EMI5), partial CSB-YP399515LLV
CSB-EP399515LLV
CSB-BP399515LLV
CSB-MP399515LLV
CSB-EP399515LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP399516LLV
CSB-EP399516LLV
CSB-BP399516LLV
CSB-MP399516LLV
CSB-EP399516LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Cx9C motif-containing protein 4, mitochondrial (CMC4) CSB-YP399517LLV
CSB-EP399517LLV
CSB-BP399517LLV
CSB-MP399517LLV
CSB-EP399517LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Sensitive to high expression protein 9 homolog, mitochondrial (SHE9), partial CSB-YP399518LLV
CSB-EP399518LLV
CSB-BP399518LLV
CSB-MP399518LLV
CSB-EP399518LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP399519LLV
CSB-EP399519LLV
CSB-BP399519LLV
CSB-MP399519LLV
CSB-EP399519LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Polyadenylate-binding protein, cytoplasmic and nuclear (PAB1), partial CSB-YP399520LLV
CSB-EP399520LLV
CSB-BP399520LLV
CSB-MP399520LLV
CSB-EP399520LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Nucleolar protein 9 (NOP9), partial CSB-YP399521LLV
CSB-EP399521LLV
CSB-BP399521LLV
CSB-MP399521LLV
CSB-EP399521LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Nuclear protein localization protein 4 (NPL4), partial CSB-YP399522LLV
CSB-EP399522LLV
CSB-BP399522LLV
CSB-MP399522LLV
CSB-EP399522LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Protein transport protein SEC13 (SEC13) CSB-YP399523LLV
CSB-EP399523LLV
CSB-BP399523LLV
CSB-MP399523LLV
CSB-EP399523LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Regulator of rDNA transcription 14 (RRT14) CSB-YP399524LLV
CSB-EP399524LLV
CSB-BP399524LLV
CSB-MP399524LLV
CSB-EP399524LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Mitochondrial inner membrane protease ATP23 (ATP23) CSB-YP399525LLV
CSB-EP399525LLV
CSB-BP399525LLV
CSB-MP399525LLV
CSB-EP399525LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Increased recombination centers protein 22 (IRC22), partial CSB-YP399526LLV
CSB-EP399526LLV
CSB-BP399526LLV
CSB-MP399526LLV
CSB-EP399526LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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