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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. Putative arginyl-tRNA--protein transferase (ate) CSB-YP399567BPE
CSB-EP399567BPE
CSB-BP399567BPE
CSB-MP399567BPE
CSB-EP399567BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. SsrA-binding protein (smpB) CSB-YP399568BPE
CSB-EP399568BPE
CSB-BP399568BPE
CSB-MP399568BPE
CSB-EP399568BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L17 (rplQ) CSB-YP399569BPE
CSB-EP399569BPE
CSB-BP399569BPE
CSB-MP399569BPE
CSB-EP399569BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S8 (rpsH) CSB-YP399570BPE
CSB-EP399570BPE
CSB-BP399570BPE
CSB-MP399570BPE
CSB-EP399570BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L14 (rplN) CSB-YP399571BPE
CSB-EP399571BPE
CSB-BP399571BPE
CSB-MP399571BPE
CSB-EP399571BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S19 (rpsS) CSB-YP399572BPE
CSB-EP399572BPE
CSB-BP399572BPE
CSB-MP399572BPE
CSB-EP399572BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L1 (rplA) CSB-YP399573BPE
CSB-EP399573BPE
CSB-BP399573BPE
CSB-MP399573BPE
CSB-EP399573BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0391 membrane protein BBta_5399 (BBta_5399), partial CSB-YP399574BPE
CSB-EP399574BPE
CSB-BP399574BPE
CSB-MP399574BPE
CSB-EP399574BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Flagellar P-ring protein (flgI) CSB-YP399575BPE
CSB-EP399575BPE
CSB-BP399575BPE
CSB-MP399575BPE
CSB-EP399575BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ketol-acid reductoisomerase (ilvC) CSB-YP399576BPE
CSB-EP399576BPE
CSB-BP399576BPE
CSB-MP399576BPE
CSB-EP399576BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP399577BPE
CSB-EP399577BPE
CSB-BP399577BPE
CSB-MP399577BPE
CSB-EP399577BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Potassium-transporting ATPase C chain (kdpC), partial CSB-YP399578BPE
CSB-EP399578BPE
CSB-BP399578BPE
CSB-MP399578BPE
CSB-EP399578BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Light-independent protochlorophyllide reductase subunit N (bchN) CSB-YP399579BPE
CSB-EP399579BPE
CSB-BP399579BPE
CSB-MP399579BPE
CSB-EP399579BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP399580BPE
CSB-EP399580BPE
CSB-BP399580BPE
CSB-MP399580BPE
CSB-EP399580BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Urease accessory protein UreF (ureF) CSB-YP399581BPE
CSB-EP399581BPE
CSB-BP399581BPE
CSB-MP399581BPE
CSB-EP399581BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Carboxylate-amine ligase BBta_7205 (BBta_7205) CSB-YP399582BPE
CSB-EP399582BPE
CSB-BP399582BPE
CSB-MP399582BPE
CSB-EP399582BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP399583BPE
CSB-EP399583BPE
CSB-BP399583BPE
CSB-MP399583BPE
CSB-EP399583BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Recombination protein RecR (recR) CSB-YP399584BPE
CSB-EP399584BPE
CSB-BP399584BPE
CSB-MP399584BPE
CSB-EP399584BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0059 membrane protein BBta_7389 (BBta_7389), partial CSB-YP399585BPE1
CSB-EP399585BPE1
CSB-BP399585BPE1
CSB-MP399585BPE1
CSB-EP399585BPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. [Protein-PII] uridylyltransferase (glnD), partial CSB-YP399586BPE
CSB-EP399586BPE
CSB-BP399586BPE
CSB-MP399586BPE
CSB-EP399586BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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