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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio cholerae serotype O1 Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP399667VEY
CSB-EP399667VEY
CSB-BP399667VEY
CSB-MP399667VEY
CSB-EP399667VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cytosol aminopeptidase (pepA), partial CSB-YP399668VEY
CSB-EP399668VEY
CSB-BP399668VEY
CSB-MP399668VEY
CSB-EP399668VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UPF0149 protein VC0395_A2053/VC395_2591 (VC0395_A2053, VC395_2591) CSB-YP399669VEY
CSB-EP399669VEY
CSB-BP399669VEY
CSB-MP399669VEY
CSB-EP399669VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP399670VEY
CSB-EP399670VEY
CSB-BP399670VEY
CSB-MP399670VEY
CSB-EP399670VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Vitamin B12-binding protein (btuF) CSB-YP399671VEY
CSB-EP399671VEY
CSB-BP399671VEY
CSB-MP399671VEY
CSB-EP399671VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable endonuclease 4 (nfo) CSB-YP399672VEY
CSB-EP399672VEY
CSB-BP399672VEY
CSB-MP399672VEY
CSB-EP399672VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP399673VEY
CSB-EP399673VEY
CSB-BP399673VEY
CSB-MP399673VEY
CSB-EP399673VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Na (+)-translocating NADH-quinone reductase subunit D, partial CSB-YP399674VEY1
CSB-EP399674VEY1
CSB-BP399674VEY1
CSB-MP399674VEY1
CSB-EP399674VEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 30S ribosomal protein S2 (rpsB) CSB-YP399675VEY
CSB-EP399675VEY
CSB-BP399675VEY
CSB-MP399675VEY
CSB-EP399675VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Ribonuclease HII (rnhB) CSB-YP399676VEY
CSB-EP399676VEY
CSB-BP399676VEY
CSB-MP399676VEY
CSB-EP399676VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Vibriobactin utilization protein ViuB (viuB) CSB-YP399677VEY
CSB-EP399677VEY
CSB-BP399677VEY
CSB-MP399677VEY
CSB-EP399677VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP399678VEY
CSB-EP399678VEY
CSB-BP399678VEY
CSB-MP399678VEY
CSB-EP399678VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Flagellin D (flaD) CSB-YP399679VEY
CSB-EP399679VEY
CSB-BP399679VEY
CSB-MP399679VEY
CSB-EP399679VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein phosphatase CheZ (cheZ) CSB-YP399680VEY
CSB-EP399680VEY
CSB-BP399680VEY
CSB-MP399680VEY
CSB-EP399680VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Probable septum site-determining protein MinC (minC) CSB-YP399681VEY
CSB-EP399681VEY
CSB-BP399681VEY
CSB-MP399681VEY
CSB-EP399681VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP399682VEY
CSB-EP399682VEY
CSB-BP399682VEY
CSB-MP399682VEY
CSB-EP399682VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Fatty acid metabolism regulator protein (fadR) CSB-YP399683VEY
CSB-EP399683VEY
CSB-BP399683VEY
CSB-MP399683VEY
CSB-EP399683VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein TolB (tolB) CSB-YP399684VEY
CSB-EP399684VEY
CSB-BP399684VEY
CSB-MP399684VEY
CSB-EP399684VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP399685VEY
CSB-EP399685VEY
CSB-BP399685VEY
CSB-MP399685VEY
CSB-EP399685VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Chromosome partition protein MukB (mukB), partial CSB-YP399686VEY
CSB-EP399686VEY
CSB-BP399686VEY
CSB-MP399686VEY
CSB-EP399686VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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