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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L24 (rplX) CSB-YP400667SUJ
CSB-EP400667SUJ
CSB-BP400667SUJ
CSB-MP400667SUJ
CSB-EP400667SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L29 (rpmC) CSB-YP400668SUJ
CSB-EP400668SUJ
CSB-BP400668SUJ
CSB-MP400668SUJ
CSB-EP400668SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L4 (rplD) CSB-YP400669SUJ
CSB-EP400669SUJ
CSB-BP400669SUJ
CSB-MP400669SUJ
CSB-EP400669SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit alpha (ureC), partial CSB-YP400670SUJ
CSB-EP400670SUJ
CSB-BP400670SUJ
CSB-MP400670SUJ
CSB-EP400670SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative 3-methyladenine DNA glycosylase (SaurJH9_2368) CSB-YP400671SUJ
CSB-EP400671SUJ
CSB-BP400671SUJ
CSB-MP400671SUJ
CSB-EP400671SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Oxygen sensor histidine kinase nreB (nreB) CSB-YP400672SUJ
CSB-EP400672SUJ
CSB-BP400672SUJ
CSB-MP400672SUJ
CSB-EP400672SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system glucoside-specific EIICBA component (glcB), partial CSB-YP400673SUJ
CSB-EP400673SUJ
CSB-BP400673SUJ
CSB-MP400673SUJ
CSB-EP400673SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP400674SUJ
CSB-EP400674SUJ
CSB-BP400674SUJ
CSB-MP400674SUJ
CSB-EP400674SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP400675SUJ
CSB-EP400675SUJ
CSB-BP400675SUJ
CSB-MP400675SUJ
CSB-EP400675SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0176 protein SaurJH9_2713 (SaurJH9_2713) CSB-YP400676SUJ
CSB-EP400676SUJ
CSB-BP400676SUJ
CSB-MP400676SUJ
CSB-EP400676SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L32 (rpmF) CSB-YP400677MWC
CSB-EP400677MWC
CSB-BP400677MWC
CSB-MP400677MWC
CSB-EP400677MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP400678MWC
CSB-EP400678MWC
CSB-BP400678MWC
CSB-MP400678MWC
CSB-EP400678MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L9 (rplI) CSB-YP400679MWC
CSB-EP400679MWC
CSB-BP400679MWC
CSB-MP400679MWC
CSB-EP400679MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae ATP synthase gamma chain (atpG) CSB-YP400680MWC
CSB-EP400680MWC
CSB-BP400680MWC
CSB-MP400680MWC
CSB-EP400680MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L17 (rplQ) CSB-YP400681MWC
CSB-EP400681MWC
CSB-BP400681MWC
CSB-MP400681MWC
CSB-EP400681MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 30S ribosomal protein S8 (rpsH) CSB-YP400682MWC
CSB-EP400682MWC
CSB-BP400682MWC
CSB-MP400682MWC
CSB-EP400682MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L29 (rpmC) CSB-YP400683MWC
CSB-EP400683MWC
CSB-BP400683MWC
CSB-MP400683MWC
CSB-EP400683MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L3 (rplC) CSB-YP400684MWC
CSB-EP400684MWC
CSB-BP400684MWC
CSB-MP400684MWC
CSB-EP400684MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae 50S ribosomal protein L7/L12 (rplL) CSB-YP400685MWC
CSB-EP400685MWC
CSB-BP400685MWC
CSB-MP400685MWC
CSB-EP400685MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae SsrA-binding protein (smpB) CSB-YP400686MWC
CSB-EP400686MWC
CSB-BP400686MWC
CSB-MP400686MWC
CSB-EP400686MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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