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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium sp. 50S ribosomal protein L2 (rplB) CSB-YP399867BPE
CSB-EP399867BPE
CSB-BP399867BPE
CSB-MP399867BPE
CSB-EP399867BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 60 kDa chaperonin 4 (groL4), partial CSB-YP399868BPE
CSB-EP399868BPE
CSB-BP399868BPE
CSB-MP399868BPE
CSB-EP399868BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ferredoxin--NADP reductase (BBta_5550) CSB-YP399869BPE
CSB-EP399869BPE
CSB-BP399869BPE
CSB-MP399869BPE
CSB-EP399869BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. S-adenosylmethionine synthase (metK) CSB-YP399870BPE
CSB-EP399870BPE
CSB-BP399870BPE
CSB-MP399870BPE
CSB-EP399870BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Thiazole synthase (thiG) CSB-YP399871BPE
CSB-EP399871BPE
CSB-BP399871BPE
CSB-MP399871BPE
CSB-EP399871BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Catalase-peroxidase (katG), partial CSB-YP399872BPE
CSB-EP399872BPE
CSB-BP399872BPE
CSB-MP399872BPE
CSB-EP399872BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0335 protein BBta_6866 (BBta_6866) CSB-YP399873BPE
CSB-EP399873BPE
CSB-BP399873BPE
CSB-MP399873BPE
CSB-EP399873BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L31 (rpmE) CSB-YP399874BPE
CSB-EP399874BPE
CSB-BP399874BPE
CSB-MP399874BPE
CSB-EP399874BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Glycerol kinase (glpK) CSB-YP399875BPE
CSB-EP399875BPE
CSB-BP399875BPE
CSB-MP399875BPE
CSB-EP399875BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP399876BPE
CSB-EP399876BPE
CSB-BP399876BPE
CSB-MP399876BPE
CSB-EP399876BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP399877BPE
CSB-EP399877BPE
CSB-BP399877BPE
CSB-MP399877BPE
CSB-EP399877BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-dehydroquinate synthase (aroB) CSB-YP399878BPE
CSB-EP399878BPE
CSB-BP399878BPE
CSB-MP399878BPE
CSB-EP399878BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP399879BPE
CSB-EP399879BPE
CSB-BP399879BPE
CSB-MP399879BPE
CSB-EP399879BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S20 (rpsT) CSB-YP399880BPE
CSB-EP399880BPE
CSB-BP399880BPE
CSB-MP399880BPE
CSB-EP399880BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Glutamate--tRNA ligase (gltX) CSB-YP399881DHZ
CSB-EP399881DHZ
CSB-BP399881DHZ
CSB-MP399881DHZ
CSB-EP399881DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP399882DHZ
CSB-EP399882DHZ
CSB-BP399882DHZ
CSB-MP399882DHZ
CSB-EP399882DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Ribonuclease PH (rph) CSB-YP399883DHZ
CSB-EP399883DHZ
CSB-BP399883DHZ
CSB-MP399883DHZ
CSB-EP399883DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Threonine--tRNA ligase (thrS), partial CSB-YP399884DHZ
CSB-EP399884DHZ
CSB-BP399884DHZ
CSB-MP399884DHZ
CSB-EP399884DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus 50S ribosomal protein L20 (rplT) CSB-YP399885DHZ
CSB-EP399885DHZ
CSB-BP399885DHZ
CSB-MP399885DHZ
CSB-EP399885DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Aspartate carbamoyltransferase (pyrB) CSB-YP399886DHZ
CSB-EP399886DHZ
CSB-BP399886DHZ
CSB-MP399886DHZ
CSB-EP399886DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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