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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP400867SVB
CSB-EP400867SVB
CSB-BP400867SVB
CSB-MP400867SVB
CSB-EP400867SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP400868SVB
CSB-EP400868SVB
CSB-BP400868SVB
CSB-MP400868SVB
CSB-EP400868SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 33 kDa chaperonin (hslO) CSB-YP400869SVB
CSB-EP400869SVB
CSB-BP400869SVB
CSB-MP400869SVB
CSB-EP400869SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP400870SVB
CSB-EP400870SVB
CSB-BP400870SVB
CSB-MP400870SVB
CSB-EP400870SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP400871SVB
CSB-EP400871SVB
CSB-BP400871SVB
CSB-MP400871SVB
CSB-EP400871SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 5'-nucleotidase surE (surE) CSB-YP400872SVB
CSB-EP400872SVB
CSB-BP400872SVB
CSB-MP400872SVB
CSB-EP400872SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable transcriptional regulatory protein SynRCC307_1833 (SynRCC307_1833) CSB-YP400873SVB
CSB-EP400873SVB
CSB-BP400873SVB
CSB-MP400873SVB
CSB-EP400873SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP400874SVB
CSB-EP400874SVB
CSB-BP400874SVB
CSB-MP400874SVB
CSB-EP400874SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome maturation factor RimP (rimP) CSB-YP400875SVB
CSB-EP400875SVB
CSB-BP400875SVB
CSB-MP400875SVB
CSB-EP400875SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L3 (rplC) CSB-YP400876SVB
CSB-EP400876SVB
CSB-BP400876SVB
CSB-MP400876SVB
CSB-EP400876SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S17 (rpsQ) CSB-YP400877SVB
CSB-EP400877SVB
CSB-BP400877SVB
CSB-MP400877SVB
CSB-EP400877SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L6 (rplF) CSB-YP400878SVB
CSB-EP400878SVB
CSB-BP400878SVB
CSB-MP400878SVB
CSB-EP400878SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoglucosamine mutase (glmM) CSB-YP400879SVB
CSB-EP400879SVB
CSB-BP400879SVB
CSB-MP400879SVB
CSB-EP400879SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (SynRCC307_2256) CSB-YP400880SVB
CSB-EP400880SVB
CSB-BP400880SVB
CSB-MP400880SVB
CSB-EP400880SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP400881SVB
CSB-EP400881SVB
CSB-BP400881SVB
CSB-MP400881SVB
CSB-EP400881SVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Peroxidase 70 (PER70) CSB-YP400882ZAX
CSB-EP400882ZAX
CSB-BP400882ZAX
CSB-MP400882ZAX
CSB-EP400882ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Peroxidase 1 (PER1) CSB-YP400883ZAX
CSB-EP400883ZAX
CSB-BP400883ZAX
CSB-MP400883ZAX
CSB-EP400883ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase Cfr (cfr) CSB-YP400884FKZ
CSB-EP400884FKZ
CSB-BP400884FKZ
CSB-MP400884FKZ
CSB-EP400884FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Recombination protein RecR (recR) CSB-YP400885CWV
CSB-EP400885CWV
CSB-BP400885CWV
CSB-MP400885CWV
CSB-EP400885CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Release factor glutamine methyltransferase (prmC) CSB-YP400886CWV
CSB-EP400886CWV
CSB-BP400886CWV
CSB-MP400886CWV
CSB-EP400886CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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