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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Transmembrane protein 150C (TMEM150C), partial CSB-YP399767BO1
CSB-EP399767BO1
CSB-BP399767BO1
CSB-MP399767BO1
CSB-EP399767BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Heat shock 70 kDa protein 14-A (hspa14-a), partial CSB-YP399768XBE
CSB-EP399768XBE
CSB-BP399768XBE
CSB-MP399768XBE
CSB-EP399768XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein HEXIM (hexim) CSB-YP399769DIL
CSB-EP399769DIL
CSB-BP399769DIL
CSB-MP399769DIL
CSB-EP399769DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Zinc finger RAD18 domain-containing protein C1orf124 homolog CSB-YP399770BO
CSB-EP399770BO
CSB-BP399770BO
CSB-MP399770BO
CSB-EP399770BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Metacaspase-1 (MCA1), partial CSB-YP399771MTM
CSB-EP399771MTM
CSB-BP399771MTM
CSB-MP399771MTM
CSB-EP399771MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii PAB-dependent poly (A)-specific ribonuclease subunit PAN2, partial CSB-YP399772MTM
CSB-EP399772MTM
CSB-BP399772MTM
CSB-MP399772MTM
CSB-EP399772MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP399773MTM
CSB-EP399773MTM
CSB-BP399773MTM
CSB-MP399773MTM
CSB-EP399773MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP399774MTM
CSB-EP399774MTM
CSB-BP399774MTM
CSB-MP399774MTM
CSB-EP399774MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Histone H2B.2 (HTB2) CSB-YP399775MTM
CSB-EP399775MTM
CSB-BP399775MTM
CSB-MP399775MTM
CSB-EP399775MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Putative lipase ATG15 (ATG15), partial CSB-YP399776MTM
CSB-EP399776MTM
CSB-BP399776MTM
CSB-MP399776MTM
CSB-EP399776MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Autophagy-related protein 27 (ATG27), partial CSB-YP399777MTM
CSB-EP399777MTM
CSB-BP399777MTM
CSB-MP399777MTM
CSB-EP399777MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Probable cysteine protease ATG4 (ATG4) CSB-YP399778MTM
CSB-EP399778MTM
CSB-BP399778MTM
CSB-MP399778MTM
CSB-EP399778MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Increased recombination centers protein 22 (IRC22), partial CSB-YP399779MTM
CSB-EP399779MTM
CSB-BP399779MTM
CSB-MP399779MTM
CSB-EP399779MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP399780MTM
CSB-EP399780MTM
CSB-BP399780MTM
CSB-MP399780MTM
CSB-EP399780MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Oxidant-induced cell-cycle arrest protein 5 (OCA5), partial CSB-YP399781MTM
CSB-EP399781MTM
CSB-BP399781MTM
CSB-MP399781MTM
CSB-EP399781MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii ATP-dependent rRNA helicase SPB4 (SPB4), partial CSB-YP399782MTM
CSB-EP399782MTM
CSB-BP399782MTM
CSB-MP399782MTM
CSB-EP399782MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1) CSB-YP399783MTM
CSB-EP399783MTM
CSB-BP399783MTM
CSB-MP399783MTM
CSB-EP399783MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Probable cytosolic iron-sulfur protein assembly protein 1 (CIA1) CSB-YP399784MTM
CSB-EP399784MTM
CSB-BP399784MTM
CSB-MP399784MTM
CSB-EP399784MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Autophagy-related protein 16 (ATG16) CSB-YP399785MTM
CSB-EP399785MTM
CSB-BP399785MTM
CSB-MP399785MTM
CSB-EP399785MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Adenylosuccinate synthetase (PGUG_03145) CSB-YP399786MTM
CSB-EP399786MTM
CSB-BP399786MTM
CSB-MP399786MTM
CSB-EP399786MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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