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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP401367SUJ
CSB-EP401367SUJ
CSB-BP401367SUJ
CSB-MP401367SUJ
CSB-EP401367SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP401368SUJ
CSB-EP401368SUJ
CSB-BP401368SUJ
CSB-MP401368SUJ
CSB-EP401368SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP401369SUJ
CSB-EP401369SUJ
CSB-BP401369SUJ
CSB-MP401369SUJ
CSB-EP401369SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal protein L11 methyltransferase (prmA) CSB-YP401370SUJ
CSB-EP401370SUJ
CSB-BP401370SUJ
CSB-MP401370SUJ
CSB-EP401370SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine--tRNA ligase (alaS), partial CSB-YP401371SUJ
CSB-EP401371SUJ
CSB-BP401371SUJ
CSB-MP401371SUJ
CSB-EP401371SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP401372SUJ
CSB-EP401372SUJ
CSB-BP401372SUJ
CSB-MP401372SUJ
CSB-EP401372SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP401373SUJ
CSB-EP401373SUJ
CSB-BP401373SUJ
CSB-MP401373SUJ
CSB-EP401373SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-adenosylmethionine synthase (metK) CSB-YP401374SUJ
CSB-EP401374SUJ
CSB-BP401374SUJ
CSB-MP401374SUJ
CSB-EP401374SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacylglycerol kinase (dagK) CSB-YP401375SUJ
CSB-EP401375SUJ
CSB-BP401375SUJ
CSB-MP401375SUJ
CSB-EP401375SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP401376SUJ
CSB-EP401376SUJ
CSB-BP401376SUJ
CSB-MP401376SUJ
CSB-EP401376SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP401377SUJ
CSB-EP401377SUJ
CSB-BP401377SUJ
CSB-MP401377SUJ
CSB-EP401377SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP401378SUJ
CSB-EP401378SUJ
CSB-BP401378SUJ
CSB-MP401378SUJ
CSB-EP401378SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP401379SUJ
CSB-EP401379SUJ
CSB-BP401379SUJ
CSB-MP401379SUJ
CSB-EP401379SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L31 type B (rpmE2) CSB-YP401380SUJ
CSB-EP401380SUJ
CSB-BP401380SUJ
CSB-MP401380SUJ
CSB-EP401380SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Galactose-6-phosphate isomerase subunit lacA (lacA) CSB-YP401381SUJ
CSB-EP401381SUJ
CSB-BP401381SUJ
CSB-MP401381SUJ
CSB-EP401381SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S8 (rpsH) CSB-YP401382SUJ
CSB-EP401382SUJ
CSB-BP401382SUJ
CSB-MP401382SUJ
CSB-EP401382SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L2 (rplB) CSB-YP401383SUJ
CSB-EP401383SUJ
CSB-BP401383SUJ
CSB-MP401383SUJ
CSB-EP401383SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP401384SUJ
CSB-EP401384SUJ
CSB-BP401384SUJ
CSB-MP401384SUJ
CSB-EP401384SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreE (ureE) CSB-YP401385SUJ
CSB-EP401385SUJ
CSB-BP401385SUJ
CSB-MP401385SUJ
CSB-EP401385SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP401386SUJ
CSB-EP401386SUJ
CSB-BP401386SUJ
CSB-MP401386SUJ
CSB-EP401386SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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