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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Legionella pneumophila Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP400567LLJ
CSB-EP400567LLJ
CSB-BP400567LLJ
CSB-MP400567LLJ
CSB-EP400567LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Probable rRNA maturation factor (LPC_0856) CSB-YP400568LLJ
CSB-EP400568LLJ
CSB-BP400568LLJ
CSB-MP400568LLJ
CSB-EP400568LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Aspartate--tRNA ligase (aspS), partial CSB-YP400569LLJ
CSB-EP400569LLJ
CSB-BP400569LLJ
CSB-MP400569LLJ
CSB-EP400569LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila tRNA pseudouridine synthase D (truD) CSB-YP400570LLJ
CSB-EP400570LLJ
CSB-BP400570LLJ
CSB-MP400570LLJ
CSB-EP400570LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP400571LLJ
CSB-EP400571LLJ
CSB-BP400571LLJ
CSB-MP400571LLJ
CSB-EP400571LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila DNA mismatch repair protein MutS (mutS), partial CSB-YP400572LLJ
CSB-EP400572LLJ
CSB-BP400572LLJ
CSB-MP400572LLJ
CSB-EP400572LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP400573LLJ
CSB-EP400573LLJ
CSB-BP400573LLJ
CSB-MP400573LLJ
CSB-EP400573LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP400574LLJ
CSB-EP400574LLJ
CSB-BP400574LLJ
CSB-MP400574LLJ
CSB-EP400574LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP400575LLJ
CSB-EP400575LLJ
CSB-BP400575LLJ
CSB-MP400575LLJ
CSB-EP400575LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP400576LLJ
CSB-EP400576LLJ
CSB-BP400576LLJ
CSB-MP400576LLJ
CSB-EP400576LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila UPF0391 membrane protein LPC_1949 (LPC_1949), partial CSB-YP400577LLJ
CSB-EP400577LLJ
CSB-BP400577LLJ
CSB-MP400577LLJ
CSB-EP400577LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 3-dehydroquinate synthase (aroB) CSB-YP400578LLJ
CSB-EP400578LLJ
CSB-BP400578LLJ
CSB-MP400578LLJ
CSB-EP400578LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Peptide chain release factor 3 (prfC), partial CSB-YP400579LLJ
CSB-EP400579LLJ
CSB-BP400579LLJ
CSB-MP400579LLJ
CSB-EP400579LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Carbon storage regulator homolog (csrA) CSB-YP400580LLJ
CSB-EP400580LLJ
CSB-BP400580LLJ
CSB-MP400580LLJ
CSB-EP400580LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L31 (rpmE) CSB-YP400581LLJ
CSB-EP400581LLJ
CSB-BP400581LLJ
CSB-MP400581LLJ
CSB-EP400581LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Aspartate carbamoyltransferase (pyrB) CSB-YP400582LLJ
CSB-EP400582LLJ
CSB-BP400582LLJ
CSB-MP400582LLJ
CSB-EP400582LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Adenylosuccinate synthetase (purA) CSB-YP400583LLJ
CSB-EP400583LLJ
CSB-BP400583LLJ
CSB-MP400583LLJ
CSB-EP400583LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila Ribosome maturation factor RimM (rimM) CSB-YP400584LLJ
CSB-EP400584LLJ
CSB-BP400584LLJ
CSB-MP400584LLJ
CSB-EP400584LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 30S ribosomal protein S11 (rpsK) CSB-YP400585LLJ
CSB-EP400585LLJ
CSB-BP400585LLJ
CSB-MP400585LLJ
CSB-EP400585LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila 50S ribosomal protein L16 (rplP) CSB-YP400586LLJ
CSB-EP400586LLJ
CSB-BP400586LLJ
CSB-MP400586LLJ
CSB-EP400586LLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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