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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Flavobacterium johnsoniae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP400367FDT
CSB-EP400367FDT
CSB-BP400367FDT
CSB-MP400367FDT
CSB-EP400367FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Probable RNA 2'-phosphotransferase (kptA) CSB-YP400368FDT
CSB-EP400368FDT
CSB-BP400368FDT
CSB-MP400368FDT
CSB-EP400368FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L5 (rplE) CSB-YP400369FDT
CSB-EP400369FDT
CSB-BP400369FDT
CSB-MP400369FDT
CSB-EP400369FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 30S ribosomal protein S3 (rpsC) CSB-YP400370FDT
CSB-EP400370FDT
CSB-BP400370FDT
CSB-MP400370FDT
CSB-EP400370FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae 50S ribosomal protein L15 (rplO) CSB-YP400371FDT
CSB-EP400371FDT
CSB-BP400371FDT
CSB-MP400371FDT
CSB-EP400371FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Arginine--tRNA ligase (argS), partial CSB-YP400372FDT
CSB-EP400372FDT
CSB-BP400372FDT
CSB-MP400372FDT
CSB-EP400372FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Shikimate kinase (aroK) CSB-YP400373FDT
CSB-EP400373FDT
CSB-BP400373FDT
CSB-MP400373FDT
CSB-EP400373FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP400374DHU
CSB-EP400374DHU
CSB-BP400374DHU
CSB-MP400374DHU
CSB-EP400374DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Indole-3-glycerol phosphate synthase (trpC) CSB-YP400375DHU
CSB-EP400375DHU
CSB-BP400375DHU
CSB-MP400375DHU
CSB-EP400375DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Probable rRNA maturation factor (DehaBAV1_1181) CSB-YP400376DHU
CSB-EP400376DHU
CSB-BP400376DHU
CSB-MP400376DHU
CSB-EP400376DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. UPF0234 protein DehaBAV1_1126 (DehaBAV1_1126) CSB-YP400377DHU
CSB-EP400377DHU
CSB-BP400377DHU
CSB-MP400377DHU
CSB-EP400377DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. N utilization substance protein B homolog (nusB) CSB-YP400378DHU
CSB-EP400378DHU
CSB-BP400378DHU
CSB-MP400378DHU
CSB-EP400378DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Acetylglutamate kinase (argB) CSB-YP400379DHU
CSB-EP400379DHU
CSB-BP400379DHU
CSB-MP400379DHU
CSB-EP400379DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Acylphosphatase (acyP) CSB-YP400380DHU
CSB-EP400380DHU
CSB-BP400380DHU
CSB-MP400380DHU
CSB-EP400380DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Ribosome-binding factor A (rbfA) CSB-YP400381DHU
CSB-EP400381DHU
CSB-BP400381DHU
CSB-MP400381DHU
CSB-EP400381DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Elongation factor 4 (lepA), partial CSB-YP400382DHU
CSB-EP400382DHU
CSB-BP400382DHU
CSB-MP400382DHU
CSB-EP400382DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP400383DHU
CSB-EP400383DHU
CSB-BP400383DHU
CSB-MP400383DHU
CSB-EP400383DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. 50S ribosomal protein L20 (rplT) CSB-YP400384DHU
CSB-EP400384DHU
CSB-BP400384DHU
CSB-MP400384DHU
CSB-EP400384DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP400385DHU
CSB-EP400385DHU
CSB-BP400385DHU
CSB-MP400385DHU
CSB-EP400385DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP400386DHU
CSB-EP400386DHU
CSB-BP400386DHU
CSB-MP400386DHU
CSB-EP400386DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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