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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Cobalamin synthase (cobS), partial CSB-YP402308CWV1
CSB-EP402308CWV1
CSB-BP402308CWV1
CSB-MP402308CWV1
CSB-EP402308CWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0061 protein CBO1195/CLC_1238 (CBO1195, CLC_1238) CSB-YP402309CWV
CSB-EP402309CWV
CSB-BP402309CWV
CSB-MP402309CWV
CSB-EP402309CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0251 protein CBO1373/CLC_1408 (CBO1373, CLC_1408) CSB-YP402310CWV
CSB-EP402310CWV
CSB-BP402310CWV
CSB-MP402310CWV
CSB-EP402310CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP402311CWV
CSB-EP402311CWV
CSB-BP402311CWV
CSB-MP402311CWV
CSB-EP402311CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA mismatch repair protein MutS (mutS), partial CSB-YP402312CWV
CSB-EP402312CWV
CSB-BP402312CWV
CSB-MP402312CWV
CSB-EP402312CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3-dehydroquinate dehydratase (aroQ) CSB-YP402313CWV
CSB-EP402313CWV
CSB-BP402313CWV
CSB-MP402313CWV
CSB-EP402313CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP402314CWV
CSB-EP402314CWV
CSB-BP402314CWV
CSB-MP402314CWV
CSB-EP402314CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP402315CWV
CSB-EP402315CWV
CSB-BP402315CWV
CSB-MP402315CWV
CSB-EP402315CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP402316CWV
CSB-EP402316CWV
CSB-BP402316CWV
CSB-MP402316CWV
CSB-EP402316CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetate kinase (ackA) CSB-YP402317CWV
CSB-EP402317CWV
CSB-BP402317CWV
CSB-MP402317CWV
CSB-EP402317CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase Der (der) CSB-YP402318CWV
CSB-EP402318CWV
CSB-BP402318CWV
CSB-MP402318CWV
CSB-EP402318CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP402319CWV
CSB-EP402319CWV
CSB-BP402319CWV
CSB-MP402319CWV
CSB-EP402319CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional purine biosynthesis protein PurH (purH) CSB-YP402320CWV
CSB-EP402320CWV
CSB-BP402320CWV
CSB-MP402320CWV
CSB-EP402320CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L27 (rpmA) CSB-YP402321CWV
CSB-EP402321CWV
CSB-BP402321CWV
CSB-MP402321CWV
CSB-EP402321CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Histidine--tRNA ligase (hisS) CSB-YP402322CWV
CSB-EP402322CWV
CSB-BP402322CWV
CSB-MP402322CWV
CSB-EP402322CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Trigger factor (tig) CSB-YP402323CWV
CSB-EP402323CWV
CSB-BP402323CWV
CSB-MP402323CWV
CSB-EP402323CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP402324CWV
CSB-EP402324CWV
CSB-BP402324CWV
CSB-MP402324CWV
CSB-EP402324CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Redox-sensing transcriptional repressor rex (rex) CSB-YP402325CWV
CSB-EP402325CWV
CSB-BP402325CWV
CSB-MP402325CWV
CSB-EP402325CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative sporulation transcription regulator WhiA (whiA) CSB-YP402326CWV
CSB-EP402326CWV
CSB-BP402326CWV
CSB-MP402326CWV
CSB-EP402326CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S4 B (rpsD2) CSB-YP402327CWV
CSB-EP402327CWV
CSB-BP402327CWV
CSB-MP402327CWV
CSB-EP402327CWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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