Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus influenzae 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP403549HSE
CSB-EP403549HSE
CSB-BP403549HSE
CSB-MP403549HSE
CSB-EP403549HSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii UPF0280 protein Msm_0088 (Msm_0088) CSB-YP403550MNN
CSB-EP403550MNN
CSB-BP403550MNN
CSB-MP403550MNN
CSB-EP403550MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP403551MNN
CSB-EP403551MNN
CSB-BP403551MNN
CSB-MP403551MNN
CSB-EP403551MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii UPF0179 protein Msm_0285 (Msm_0285) CSB-YP403552MNN
CSB-EP403552MNN
CSB-BP403552MNN
CSB-MP403552MNN
CSB-EP403552MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP403553MNN
CSB-EP403553MNN
CSB-BP403553MNN
CSB-MP403553MNN
CSB-EP403553MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii V-type ATP synthase beta chain (atpB) CSB-YP403554MNN
CSB-EP403554MNN
CSB-BP403554MNN
CSB-MP403554MNN
CSB-EP403554MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP403555MNN
CSB-EP403555MNN
CSB-BP403555MNN
CSB-MP403555MNN
CSB-EP403555MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Transcription factor E (tfe) CSB-YP403556MNN
CSB-EP403556MNN
CSB-BP403556MNN
CSB-MP403556MNN
CSB-EP403556MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 30S ribosomal protein S3Ae (rps3Ae) CSB-YP403557MNN
CSB-EP403557MNN
CSB-BP403557MNN
CSB-MP403557MNN
CSB-EP403557MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 50S ribosomal protein L15P (rpl15p) CSB-YP403558MNN
CSB-EP403558MNN
CSB-BP403558MNN
CSB-MP403558MNN
CSB-EP403558MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 50S ribosomal protein L5P (rpl5p) CSB-YP403559MNN
CSB-EP403559MNN
CSB-BP403559MNN
CSB-MP403559MNN
CSB-EP403559MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 30S ribosomal protein S3P (rps3p) CSB-YP403560MNN
CSB-EP403560MNN
CSB-BP403560MNN
CSB-MP403560MNN
CSB-EP403560MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 50S ribosomal protein L3P (rpl3p) CSB-YP403561MNN
CSB-EP403561MNN
CSB-BP403561MNN
CSB-MP403561MNN
CSB-EP403561MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Translation initiation factor 5A (eIF5A) CSB-YP403562MNN
CSB-EP403562MNN
CSB-BP403562MNN
CSB-MP403562MNN
CSB-EP403562MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii FO synthase subunit 1 (cofG) CSB-YP403563MNN
CSB-EP403563MNN
CSB-BP403563MNN
CSB-MP403563MNN
CSB-EP403563MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii DNA polymerase sliding clamp (pcn) CSB-YP403564MNN
CSB-EP403564MNN
CSB-BP403564MNN
CSB-MP403564MNN
CSB-EP403564MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein (Msm_1198), partial CSB-YP403565MNN
CSB-EP403565MNN
CSB-BP403565MNN
CSB-MP403565MNN
CSB-EP403565MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Aspartate carbamoyltransferase (pyrB) CSB-YP403566MNN
CSB-EP403566MNN
CSB-BP403566MNN
CSB-MP403566MNN
CSB-EP403566MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii Glutamate--tRNA ligase (gltX), partial CSB-YP403567MNN
CSB-EP403567MNN
CSB-BP403567MNN
CSB-MP403567MNN
CSB-EP403567MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter smithii 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP403568MNN
CSB-EP403568MNN
CSB-BP403568MNN
CSB-MP403568MNN
CSB-EP403568MNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>