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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Psychrobacter sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP406311PZR
CSB-EP406311PZR
CSB-BP406311PZR
CSB-MP406311PZR
CSB-EP406311PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. 50S ribosomal protein L11 (rplK) CSB-YP406312PZR
CSB-EP406312PZR
CSB-BP406312PZR
CSB-MP406312PZR
CSB-EP406312PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Aspartate 1-decarboxylase (panD) CSB-YP406313PZR
CSB-EP406313PZR
CSB-BP406313PZR
CSB-MP406313PZR
CSB-EP406313PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP406314PZR
CSB-EP406314PZR
CSB-BP406314PZR
CSB-MP406314PZR
CSB-EP406314PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. 50S ribosomal protein L35 (rpmI) CSB-YP406315PZR
CSB-EP406315PZR
CSB-BP406315PZR
CSB-MP406315PZR
CSB-EP406315PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychrobacter sp. Chaperone protein DnaK (dnaK), partial CSB-YP406316PZR
CSB-EP406316PZR
CSB-BP406316PZR
CSB-MP406316PZR
CSB-EP406316PZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Pup--protein ligase (pafA) CSB-YP406317MOO
CSB-EP406317MOO
CSB-BP406317MOO
CSB-MP406317MOO
CSB-EP406317MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sistrurus catenatus edwardsii Toxin 3 CSB-YP406318SZP
CSB-EP406318SZP
CSB-BP406318SZP
CSB-MP406318SZP
CSB-EP406318SZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase (iolG) CSB-YP406319LMH
CSB-EP406319LMH
CSB-BP406319LMH
CSB-MP406319LMH
CSB-EP406319LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho guanine nucleotide exchange factor 35 (ARHGEF35) CSB-YP406320HU
CSB-EP406320HU
CSB-BP406320HU
CSB-MP406320HU
CSB-EP406320HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP406321GEO1
CSB-EP406321GEO1
CSB-BP406321GEO1
CSB-MP406321GEO1
CSB-EP406321GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP406322GEO
CSB-EP406322GEO
CSB-BP406322GEO
CSB-MP406322GEO
CSB-EP406322GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP406323GEO1
CSB-EP406323GEO1
CSB-BP406323GEO1
CSB-MP406323GEO1
CSB-EP406323GEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium Photosystem II reaction center protein L (psbL), partial CSB-YP406324GEO
CSB-EP406324GEO
CSB-BP406324GEO
CSB-MP406324GEO
CSB-EP406324GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gnetum parvifolium DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP406325GEO
CSB-EP406325GEO
CSB-BP406325GEO
CSB-MP406325GEO
CSB-EP406325GEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Aspartate 1-decarboxylase (panD) CSB-YP406326FDU
CSB-EP406326FDU
CSB-BP406326FDU
CSB-MP406326FDU
CSB-EP406326FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP406327FDU
CSB-EP406327FDU
CSB-BP406327FDU
CSB-MP406327FDU
CSB-EP406327FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum UvrABC system protein C (uvrC), partial CSB-YP406328FDU
CSB-EP406328FDU
CSB-BP406328FDU
CSB-MP406328FDU
CSB-EP406328FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum DNA repair protein recO (recO) CSB-YP406329FDU
CSB-EP406329FDU
CSB-BP406329FDU
CSB-MP406329FDU
CSB-EP406329FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium psychrophilum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP406330FDU
CSB-EP406330FDU
CSB-BP406330FDU
CSB-MP406330FDU
CSB-EP406330FDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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