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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus UPF0365 protein SaurJH1_1665 (SaurJH1_1665), partial CSB-YP407712SUI1
CSB-EP407712SUI1
CSB-BP407712SUI1
CSB-MP407712SUI1
CSB-EP407712SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein grpE (grpE) CSB-YP407713SUI
CSB-EP407713SUI
CSB-BP407713SUI
CSB-MP407713SUI
CSB-EP407713SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S20 (rpsT) CSB-YP407714SUI
CSB-EP407714SUI
CSB-BP407714SUI
CSB-MP407714SUI
CSB-EP407714SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uridine kinase (udk) CSB-YP407715SUI
CSB-EP407715SUI
CSB-BP407715SUI
CSB-MP407715SUI
CSB-EP407715SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L27 (rpmA) CSB-YP407716SUI
CSB-EP407716SUI
CSB-BP407716SUI
CSB-MP407716SUI
CSB-EP407716SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable GTP-binding protein EngB (engB) CSB-YP407717SUI
CSB-EP407717SUI
CSB-BP407717SUI
CSB-MP407717SUI
CSB-EP407717SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA sulfurtransferase (thiI) CSB-YP407718SUI
CSB-EP407718SUI
CSB-BP407718SUI
CSB-MP407718SUI
CSB-EP407718SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP407719SUI
CSB-EP407719SUI
CSB-BP407719SUI
CSB-MP407719SUI
CSB-EP407719SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0374 protein SaurJH1_1955 (SaurJH1_1955) CSB-YP407720SUI
CSB-EP407720SUI
CSB-BP407720SUI
CSB-MP407720SUI
CSB-EP407720SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacylglycerol kinase (dagK) CSB-YP407721SUI
CSB-EP407721SUI
CSB-BP407721SUI
CSB-MP407721SUI
CSB-EP407721SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2-isopropylmalate synthase (leuA), partial CSB-YP407722SUI
CSB-EP407722SUI
CSB-BP407722SUI
CSB-MP407722SUI
CSB-EP407722SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein sprT-like (SaurJH1_2136) CSB-YP407723SUI
CSB-EP407723SUI
CSB-BP407723SUI
CSB-MP407723SUI
CSB-EP407723SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit c (atpE), partial CSB-YP407724SUI1
CSB-EP407724SUI1
CSB-BP407724SUI1
CSB-MP407724SUI1
CSB-EP407724SUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP407725SUI
CSB-EP407725SUI
CSB-BP407725SUI
CSB-MP407725SUI
CSB-EP407725SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Galactose-6-phosphate isomerase subunit lacB (lacB) CSB-YP407726SUI
CSB-EP407726SUI
CSB-BP407726SUI
CSB-MP407726SUI
CSB-EP407726SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S13 (rpsM) CSB-YP407727SUI
CSB-EP407727SUI
CSB-BP407727SUI
CSB-MP407727SUI
CSB-EP407727SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L18 (rplR) CSB-YP407728SUI
CSB-EP407728SUI
CSB-BP407728SUI
CSB-MP407728SUI
CSB-EP407728SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L23 (rplW) CSB-YP407729SUI
CSB-EP407729SUI
CSB-BP407729SUI
CSB-MP407729SUI
CSB-EP407729SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP407730SUI
CSB-EP407730SUI
CSB-BP407730SUI
CSB-MP407730SUI
CSB-EP407730SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nitrate transporter narT (narT), partial CSB-YP407731SUI
CSB-EP407731SUI
CSB-BP407731SUI
CSB-MP407731SUI
CSB-EP407731SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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