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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium beijerinckii 50S ribosomal protein L27 (rpmA) CSB-YP407312DUC
CSB-EP407312DUC
CSB-BP407312DUC
CSB-MP407312DUC
CSB-EP407312DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Probable endonuclease 4 (nfo) CSB-YP407313DUC
CSB-EP407313DUC
CSB-BP407313DUC
CSB-MP407313DUC
CSB-EP407313DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii 30S ribosomal protein S20 (rpsT) CSB-YP407314DUC
CSB-EP407314DUC
CSB-BP407314DUC
CSB-MP407314DUC
CSB-EP407314DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Arginine--tRNA ligase (argS), partial CSB-YP407315DUC
CSB-EP407315DUC
CSB-BP407315DUC
CSB-MP407315DUC
CSB-EP407315DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Putative Holliday junction resolvase (Cbei_1106) CSB-YP407316DUC
CSB-EP407316DUC
CSB-BP407316DUC
CSB-MP407316DUC
CSB-EP407316DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP407317DUC
CSB-EP407317DUC
CSB-BP407317DUC
CSB-MP407317DUC
CSB-EP407317DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii 30S ribosomal protein S15 (rpsO) CSB-YP407318DUC
CSB-EP407318DUC
CSB-BP407318DUC
CSB-MP407318DUC
CSB-EP407318DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii S-ribosylhomocysteine lyase (luxS) CSB-YP407319DUC
CSB-EP407319DUC
CSB-BP407319DUC
CSB-MP407319DUC
CSB-EP407319DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP407320DUC
CSB-EP407320DUC
CSB-BP407320DUC
CSB-MP407320DUC
CSB-EP407320DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Dihydroxy-acid dehydratase (ilvD), partial CSB-YP407321DUC
CSB-EP407321DUC
CSB-BP407321DUC
CSB-MP407321DUC
CSB-EP407321DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP407322DUC
CSB-EP407322DUC
CSB-BP407322DUC
CSB-MP407322DUC
CSB-EP407322DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Cell division protein SepF (sepF) CSB-YP407323DUC
CSB-EP407323DUC
CSB-BP407323DUC
CSB-MP407323DUC
CSB-EP407323DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Arginine repressor (argR) CSB-YP407324DUC
CSB-EP407324DUC
CSB-BP407324DUC
CSB-MP407324DUC
CSB-EP407324DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP407325DUC
CSB-EP407325DUC
CSB-BP407325DUC
CSB-MP407325DUC
CSB-EP407325DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP407326DUC
CSB-EP407326DUC
CSB-BP407326DUC
CSB-MP407326DUC
CSB-EP407326DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP407327DUC
CSB-EP407327DUC
CSB-BP407327DUC
CSB-MP407327DUC
CSB-EP407327DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Glycerol kinase (glpK) CSB-YP407328DUC
CSB-EP407328DUC
CSB-BP407328DUC
CSB-MP407328DUC
CSB-EP407328DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii 3-dehydroquinate dehydratase (aroQ) CSB-YP407329DUC
CSB-EP407329DUC
CSB-BP407329DUC
CSB-MP407329DUC
CSB-EP407329DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii UPF0042 nucleotide-binding protein Cbei_4857 (Cbei_4857) CSB-YP407330DUC
CSB-EP407330DUC
CSB-BP407330DUC
CSB-MP407330DUC
CSB-EP407330DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP407331DUC
CSB-EP407331DUC
CSB-BP407331DUC
CSB-MP407331DUC
CSB-EP407331DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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